アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報

新型コロナウイルス (COVID-19)に伴う当社の対応について

  1. Link

    rabbit-mouse-igg-hl-alkaline-phosphatase-ab6729.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgG
Share by email

Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6729)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (21)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Rabbit
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB

こちらの製品もご検討ください

ブロッキング
Normal Rabbit Serum (ab166640)
IHC
Product image
Fast Red Substrate Kit (ab64254)
クロモゲン
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)

関連製品

製品の概要

  • 製品名

    Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
    IgG 二次抗体 製品一覧
  • 由来種

    Rabbit
  • ターゲット生物種

    Mouse
  • アプリケーション

    適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • 免疫原

    Mouse IgG whole molecule

  • 標識

    Alkaline Phosphatase

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C. Diluted stock solutions should be used immediately and not be frozen. Do Not Freeze. Please see notes section. Store undiluted.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.01% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • 精製度

    Affinity purified
  • 特記事項(精製)

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 特記事項(標識)

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • ポリ/モノ

    ポリクローナル
  • 特記事項

    Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

  • 研究分野

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Enzyme
    • Alkaline Phos

関連製品

    アプリケーション

    Our Abpromise guarantee covers the use of ab6729 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Dot blot Use at an assay dependent concentration.
    ELISA Use at an assay dependent dilution.
    IHC-P Use at an assay dependent concentration.
    IHC-Fr Use at an assay dependent concentration.
    Immunomicroscopy Use at an assay dependent concentration.
    ICC/IF 1/500 - 1/2000.
    WB Use at an assay dependent dilution.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (21)

    ab6729 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6729 は 21 報の論文で使用されています。

    • Nunes C  et al. Red wine extract preserves tight junctions in intestinal epithelial cells under inflammatory conditions: implications for intestinal inflammation. Food Funct 10:1364-1374 (2019). PubMed: 30735221
    • Kuznetsov JN  et al. BAP1 regulates epigenetic switch from pluripotency to differentiation in developmental lineages giving rise to BAP1-mutant cancers. Sci Adv 5:eaax1738 (2019). PubMed: 31555735
    • Shan X  et al. Edoxaban improves atrial fibrillation and thromboembolism through regulation of the Wnt-ß-induced PI3K/ATK-activated protein C system. Exp Ther Med 17:3509-3517 (2019). PubMed: 30988731
    • Sun AC  et al. High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array. IEEE J Solid-State Circuits 53:2054-2064 (2018). PubMed: 30559530
    • Jang J  et al. Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens. Sci Rep 8:7211 (2018). PubMed: 29739951
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

    BATCH NUMBER 131853 ORDER NUMBER MBPO/023 DESCRIPTION OF THE PROBLEM No signal of the relavant antigen (I have tried two different primary: see below), many non-target bands. SAMPLE Budding yeast cell extract (PCNA-Myc and non-tagged). PRIMARY ANTIBODY Abcam ab56 anti-Myc (9E11), Lot 130523, 1000x to 5000x dilution, 1 hr. or Abcam ab29 anti-PCNA, Lot 119707, 1000x to 5000x dilution, 1 hr. DETECTION METHOD CDP-star reagent from Perkin Elmer. POSITIVE AND NEGATIVE CONTROLS USED For detection of Myc-tagged protein, I included non-taggad sample as negative control. Size difference in Myc-tagged and non-tagged samples must help to identify correct band. Same blot has worked fine with HRP-Anti Myc from Santa Cruz, enabled me to compare the result. ANTIBODY STORAGE CONDITIONS In fridge (4 degree) w/o dilution. SAMPLE PREPARATION Roche Complete as protease inhibitor. Boiled soon after addition of SDS. AMOUNT OF PROTEIN LOADED up to 10 ug. ELECTROPHORESIS/GEL CONDITIONS Reducing, mainly 6% and 12%. TRANSFER AND BLOCKING CONDITIONS 5% milk in PBS-T as blocking. SECONDARY ANTIBODY ab6729 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I tried several different dilution of both primary and secondary (x1000 to x5000). At 1000x dilution, backgorund was very high. At 3000x or 5000x dilution, background was reduced, but no target band. Similar pattern of background bands. I also tried "No Primay". It gave quite similar background bands. ADDITIONAL NOTES I used ab6729 in combination with ab29 anti-PCNA and ab56 anti-Myc. Both gave essentially identical result (no target band, many background). The same blot worked fine for HRP-anti Myc from Santa Cruz. Also not tested in western, I believe that ab56 anti-Myc is OK, because I and a student have used it for ChIP and got nice results. Taken together, I conclude that an6729 doesn't specifically recognize mouse IgG. I prefer replacement to other antibody with good record, such as ab6790, rather than refunding.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 23 2005

    Answer

    Thank you for contacting us for technical support with ab6729. I think the problem you are experiencing is due to the fact that budding yeast expresses an endogenous form of alkaline phosphate (http://www.expasy.ch/uniprot/O60109) and by using a secondary antibody conjugated to AP this creates a background signal. I would therefore recommend using a HRP conjugated secondary antibody (and ECl + detection kit) to prevent this problem. Please do not hesitate to contact us again if you still experience problems with an HRP-conjugated secondary antibody,

    Read More

    Abcam Scientific Support

    Answered on Nov 25 2005

    Question

    The lot # is: 71496 and the order number: 68495 Thanks a lot.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2005

    Answer

    I have heard back from the originator of this product and would like to pass along the following comments to you (possible resaons why the AP loss activity). a). Researcher diluted the product and stored at 4oC. THE PRODUCT HAS TO BE STORED UNDILUTED. b). The product was accidently frozen, resulting in reduced activity. c). The pipette used for sampling was contiminated, that may accelarate AP degredation. That often happens. If you touched the tips, they may have been contaminated with DNAse, proteases etc. Also AP should be stored in dark tubes. d). During storage, the liquid evaporated to the cap, causing the antibody to dry. e). The substrate was at the wrong pH, or went off, so reaction was not at optimal conditions. I just wanted to pass this onto to you, please let me know if you would like a replacement vial of ab6729.

    Read More

    Abcam Scientific Support

    Answered on Apr 01 2005

    Question

    The lot # is: 71496 and the order number: 68495 Thanks a lot.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2005

    Answer

    Thank you for your email. I have contacted the originator of this antibody in order to see if they have any suggestions or have heard of any problems with the particular lot that you have received. I will not hear back until next week and I'm also going to be away next week. I will pass their comments onto you when I do hear. In the meantime, if you would like to try a replacement vial, please let me know and I or one of my colleagues will arrange that for you. Thank you again, and have a great weekend.

    Read More

    Abcam Scientific Support

    Answered on Apr 01 2005

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM loss of activity of the alkaline phosphatase SAMPLE amyloid fibers from a-beta: used anti-abeta antibody and than used the anti mouse IgG as secondary antibody PRIMARY ANTIBODY Abcam ab 10146 dilution 1:6000 SECONDARY ANTIBODY abcam ab 6729 dilution 1:1000 DETECTION METHOD UV-absorption POSITIVE AND NEGATIVE CONTROLS USED yes SAMPLE PREPARATION coated plates with amyloid, incubated with anti abeta and than with anti mouse IgG TRANSFER AND BLOCKING CONDITIONS BSA blocking HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 20 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes WHAT STEPS HAVE YOU ALTERED? incubation times and concentrations ADDITIONAL NOTES I tried the assay many times to find the right concentration, and eventually found it. the conentrations were in the described range for these antibodies. However suddenly the turnover rate of the alkaline phophatase came to almost a stand still within two weeks of using it. Normally is took 30 - 45 minutes to get the desired response. Now it takes 15-20 hours to get a comparable response, with the same time and concentrations used before. does the alkaline phosphase decompose after a certain period of time? I kept it at 4 degrees as was said in the data sheet.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 28 2005

    Answer

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab6729. Can you please tell me the lot number that you received (it is located on the vial) and the Abcam order number or purchase order number that was used?

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2005

    Question

    >lot 52644 (My PO no: 04-12-0141) Abcam Reference No: 64641 (Invoice No: 48594) > > ab8226-200 > > >1. Please describe the problem (high background, wrong band size, more bands, no band etc). > >2. On what material are you testing the antibody in WB? >.Species? Rabbit muscle tissue >.Cell extract/ Nuclear extract? Tissue extract >.Purified protein? >.Recombinant protein? > >3. How much protein did you load? 10ug >.How did you prepare the lysate for the analysis (protease inhibitors etc)? sonicate with lysis buffer >.Did you heat the samples? yes > >4. Primary Antibody >.Specification (in which species was it raised against)? ab8226-200 >.At what dilution(s) have you tested this antibody? 1:250 >.Incubation time, wash step? 1 hr , wash - 5min, 15min, 5 min and 5 min > >5. Secondary Antibody >.Specification (in which species was it raised against)? ab 6729 >.At what dilution(s) have you tested this antibody? 1:5000 >.Incubation time, wash step? 1 hr, wash - 15min, 5 min 4 times >.Do you know whether the problems you are experiencing come from the secondary? No, other antibodies are within the specify range from supplier > >6. What detection method are you using? Perkin Elmer western Lightening chemiluminescence > >7. Background bands >.Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a "No primary" control) >.Is the blocking step sufficient? Yes (we use 0.3g skimmed milk, 5ul tween 20 in 50 ml TBS.) > > .Are your washing steps sufficiently stringent? (Multiple short washes are more effective than fewer longer wash steps) >.At what size are the bands migrating? Could they be degradation products of your target? Probably > >8. Optimization attempts >.How many times have you tried the Western? 4 times >.Do you obtain the same results every time e.g. are background bands always in the same place? I don't get any bands with the recommended dilution on the data specification sheet I get bands only when I use 1 :250 dilution. >.What steps have you altered? primary antibody dilution I have tried 1:10000, 1:1000, 1:500 and 1:250 (only the 1:250 dilution give bands) > >9. Did you apply positive and negative controls along with the samples? Please specify. No

    Read More

    Abcam community

    Verified customer

    Asked on Feb 15 2005

    Answer

    Thank you for the details that you have provided. I'm surprised that you are unable to see a signal with ab8226 except for the faint signal detected with the dilution of 1:250. We have had some good feedback from customers who have successfully used the antibody at dilutions from 1:1000 to 1:5000. I can certainly send you a replacement vial of ab8226 free of charge. Please let me know if you are interested.

    Read More

    Abcam Scientific Support

    Answered on Feb 16 2005

    Question

    I have an enquiry which I don't know whether other of your customer faces it. I had bought ab8226 (Anti beta actin) a few weeks ago and according to the data sheet the recommended dilution for westtern blot is between 1/500-1/10000. I have tried with 1/10000 with no bands and 1/500 with super faint band after 1 hour exposure, then I tried with 1/250 and exposed for 1 hour and I get better result, the blot is attached. I am using Ab6729 (diltuon 1/5000) as secondary antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2005

    Answer

    Thanks for your email and I'm sorry to hear that you are experiencing difficulty with ab8226. To better assist you, can you please provide me with me with more details regarding your protocol? Also, please include the lot number that you received (it is located on the vial) and the Abcam order number or purchase order number that was used. Thank you, and I look forward to hearing from you. 1. Please describe the problem (high background, wrong band size, more bands, no band etc). 2. On what material are you testing the antibody in WB? •Species? •Cell extract/ Nuclear extract? •Purified protein? •Recombinant protein? 3. How much protein did you load? •How did you prepare the lysate for the analysis (protease inhibitors etc)? •Did you heat the samples? 4. Primary Antibody •Specification (in which species was it raised against)? •At what dilution(s) have you tested this antibody? •Incubation time, wash step? 5. Secondary Antibody •Specification (in which species was it raised against)? •At what dilution(s) have you tested this antibody? •Incubation time, wash step? •Do you know whether the problems you are experiencing come from the secondary? 6. What detection method are you using? 7. Background bands •Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control) •Is the blocking step sufficient? (We recommend blocking the membrane by adding 20 ml of blocking buffer (5% non-fat dry milk, 0.1% Tween-20 in TBS). Incubate for 2 h at room temperature or overnight at 4°C with agitation) •Are your washing steps sufficiently stringent? (Multiple short washes are more effective than fewer longer wash steps) •At what size are the bands migrating? Could they be degradation products of your target? •Please provide an image of your blot (as an e-mail attachment, a faxed image is not sufficient) 8. Optimization attempts •How many times have you tried the Western? •Do you obtain the same results every time e.g. are background bands always in the same place? •What steps have you altered? 9. Did you apply positive and negative controls along with the samples? Please specify.

    Read More

    Abcam Scientific Support

    Answered on Feb 07 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.