Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617)
Key features and details
- Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, WB, Sandwich ELISA
製品の概要
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製品名
Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin)
IgG4 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Human -
特異性
ab238617 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
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アプリケーション
適用あり: IHC-P, WB, Sandwich ELISAmore details -
免疫原
Synthetic peptide corresponding to Human IgG4 (Hinge).
Database link: P01861 -
標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from an animal origin–free culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
RM120 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab238617の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 - 10 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 36 kDa.
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Sandwich ELISA |
Use at an assay dependent concentration.
Use 50ng/well – 200ng/well (for Capture); 0.05µg/mL – 0.2µg/mL (for Detection). |
特記事項 |
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IHC-P
Use a concentration of 1 - 10 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 36 kDa. |
Sandwich ELISA
Use at an assay dependent concentration. Use 50ng/well – 200ng/well (for Capture); 0.05µg/mL – 0.2µg/mL (for Detection). |
画像
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Sandwich ELISA using ab238617 as the capture antibody at 200ng/well, and a Biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.
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Sandwich ELISA using ab238617 as the capture antibody, and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.
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Paraffin-embedded human lymphoid tissue stained for IgG4 heavy chain using ab238617 at 10 μg/mL in immunohistochemical analysis.
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A titer ELISA using ab238617. The plate was coated with different amounts of human IgG4. A serial dilution of ab238617 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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All lanes : Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617) at 2 µg/ml
Lane 1 : Non-reduced (-) and reduced (+) forms of Human IgG1
Lane 2 : Non-reduced (-) and reduced (+) forms of Human IgG2
Lane 3 : Non-reduced (-) and reduced (+) forms of Human IgG3
Lane 4 : Non-reduced (-) and reduced (+) forms of Human IgG4
Lane 5 : Non-reduced (-) and reduced (+) forms of Mouse IgG
Lane 6 : Non-reduced (-) and reduced (+) forms of Rat IgG
Lane 7 : Non-reduced (-) and reduced (+) forms of Goat IgG
Predicted band size: 36 kDaWestern Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms (Lane 4). No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab238617 は論文での使用が確認できていません。