Rabbit monoclonal [RM118] Anti-Human IgG2 heavy chain (Biotin) (ab272184)
Key features and details
- Rabbit monoclonal [RM118] Anti-Human IgG2 heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA
製品の概要
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製品名
Rabbit monoclonal [RM118] Anti-Human IgG2 heavy chain (Biotin) -
由来種
Rabbit -
ターゲット生物種
Human -
特異性
ab272184 reacts to the heavy chain of human IgG2. No cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
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アプリケーション
適用あり: ELISAmore details -
免疫原
Full length protein corresponding to Human IgG2.
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
From an animal origin–free culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
RM118 -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
Capture: 50ng/well – 200ng/well |
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. Capture: 50ng/well – 200ng/well |
画像
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ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)
A titer ELISA using ab272184. The plate was coated with different amounts of human IgG2. A serial dilution of ab272184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)ELISA of human immunoglobulins shows ab272184 only reacted to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of ab272184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)Sandwich ELISA using ab272184 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.
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Sandwich ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)Sandwich ELISA using ab272184 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272184 は論文での使用が確認できていません。
