Rabbit monoclonal [RM117] Anti-Human IgG1 heavy chain (Biotin) (ab272183)
Key features and details
- Rabbit monoclonal [RM117] Anti-Human IgG1 heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA
製品の概要
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製品名
Rabbit monoclonal [RM117] Anti-Human IgG1 heavy chain (Biotin) -
由来種
Rabbit -
ターゲット生物種
Human -
アプリケーション
適用あり: ELISAmore details -
免疫原
Synthetic peptide corresponding to Human Human IgG (Hinge).
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
RM117 -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
Capture: 50ng/well – 200ng/well |
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. Capture: 50ng/well – 200ng/well |
画像
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ELISA - Rabbit monoclonal RM117 Anti-Human IgG1 heavy chain (Biotin) (ab272183)
A titer ELISA using ab272183. The plate was coated with different amounts of human IgG1. A serial dilution of ab272183 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA - Rabbit monoclonal RM117 Anti-Human IgG1 heavy chain (Biotin) (ab272183)ELISA of human immunoglobulins shows ab272183 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab272183 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA - Rabbit monoclonal RM117 Anti-Human IgG1 heavy chain (Biotin) (ab272183)Sandwich ELISA using ab272183 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.
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Sandwich ELISA - Rabbit monoclonal RM117 Anti-Human IgG1 heavy chain (Biotin) (ab272183)Sandwich ELISA using ab272183 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272183 は論文での使用が確認できていません。
