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リコンビナントRabMAb

Rabbit monoclonal [R18-2] Anti-Rat IgG Fc (ab125900)

Submit a review Q&A (1)References (10)
ELISA - Rabbit monoclonal [R18-2] Anti-Rat IgG Fc (ab125900)
  • ELISA - Rabbit monoclonal [R18-2] Anti-Rat IgG Fc (ab125900)

Key features and details

  • Rabbit monoclonal [R18-2] Anti-Rat IgG Fc
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: ELISA

製品の概要

  • 製品名

    Rabbit monoclonal [R18-2] Anti-Rat IgG Fc
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Rat

  • アプリケーション

    適用あり: ELISAmore details

  • 免疫原

    Other Immunogen Type corresponding to IgG.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    R18-2

  • アイソタイプ

    IgG

  • 特記事項

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab125900の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.

画像

  • ELISA - Rabbit monoclonal [R18-2] Anti-Rat IgG Fc (ab125900)
    ELISA - Rabbit monoclonal [R18-2] Anti-Rat IgG Fc (ab125900)

    Direct ELISA antibody dose-response curve using ab125900 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • ELISA - Rabbit monoclonal [R18-2] Anti-Rat IgG Fc (ab125900)
    ELISA - Rabbit monoclonal [R18-2] Anti-Rat IgG Fc (ab125900)

    Antibody dose-response curve using purified ab125900. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (10)

ab125900 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab125900 は 10 報の論文で使用されています。

  • Wang J  et al. SCARA5 suppresses the proliferation and migration, and promotes the apoptosis of human retinoblastoma cells by inhibiting the PI3K/AKT pathway. Mol Med Rep 23:N/A (2021). PubMed: 33495818
  • Ma F  et al. The microRNA-130a-5p/RUNX2/STK32A network modulates tumor invasive and metastatic potential in non-small cell lung cancer. BMC Cancer 20:580 (2020). PubMed: 32571328
  • Liu Y  et al. LncRNA MIR4435-2HG inhibits the progression of osteoarthritis through miR-510-3p sponging. Exp Ther Med 20:1693-1701 (2020). PubMed: 32742398
  • Li XF  et al. miR-363 Alleviates Detrusor Fibrosis via the TGF-ß1/Smad Signaling Pathway by Targeting Col1a2 in Rat Models of STZ-Induced T2DM. Mol Ther Nucleic Acids 22:1142-1153 (2020). PubMed: 33294298
  • Guo X & Li M LINC01089 is a tumor-suppressive lncRNA in gastric cancer and it regulates miR-27a-3p/TET1 axis. Cancer Cell Int 20:507 (2020). PubMed: 33088215
  • Ye M  et al. LPS-Inducible lncRNA TMC3-AS1 Negatively Regulates the Expression of IL-10. Front Immunol 11:1418 (2020). PubMed: 32774335
  • de Castro Martin IF  et al. Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles. Nat Commun 8:1396 (2017). PubMed: 29123131
  • Wittenborn TR  et al. Simulation of heterogeneous molecular delivery in tumours using µCT reconstructions and MRI validation. Microvasc Res 108:69-74 (2016). PubMed: 27569845
  • Fons P  et al. Tumor vasculature is regulated by FGF/FGFR signaling-mediated angiogenesis and bone marrow-derived cell recruitment: this mechanism is inhibited by SSR128129E, the first allosteric antagonist of FGFRs. J Cell Physiol 230:43-51 (2015). PubMed: 24760775
  • Hendaoui I  et al. Tenascin-C is required for normal Wnt/ß-catenin signaling in the whisker follicle stem cell niche. Matrix Biol 40:46-53 (2014). PubMed: 25196097

レビューと Q&A

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レビューを送る 質問を送る

Question

How is the specificity created and confirmed?

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Answer

Thank you very much for your call last week and for your patience while I've been in touch with the lab regarding your enquiry.
The immunogen was whole rat IgG, but different subclones were generated and then were tested for specificity against F(ab) or Fc fragments by ELISA.

I hope that this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help.

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