リコンビナントRabMAb

Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

Q&A (13)References (8)

製品の概要

  • 製品名

    Rabbit IgG, monoclonal [SP137] - isotype control
    Rabbit アイソタイプ・コントロール 製品一覧

  • アプリケーション

    適用あり: IHC-P, ICC/IF, Flow Cytmore details

  • 免疫原

    Chemical/ Small Molecule.

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Human: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C.

  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Purified from TCS by protein A/G.

  • ポリ/モノ

    モノクローナル

  • クローン名

    SP137

  • アイソタイプ

    IgG

  • 研究分野

  • 別名

    • rabbit isotype control

アプリケーション

Our Abpromise guarantee covers the use of ab125938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling Rabbit with ab125938 at 1/100 dilution (2.02 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)
    Flow Cytometry - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling EPF with ab125938 at 1:100 dilution (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab125938) at 1:20 dilution (10.1 µg/ml)/ Black. Unlabeled control - Unlabelled cells / blue.

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)
    Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells showing staining with purified ab125938 at 1:20 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling Rabbit with ab125938 at 1/100 dilution (2.02 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Rabbit with ab125938 at 1/100 dilution (2.02 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)
    Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat ( human T cell leukemia T lymphocyte) cells showing staining with purified ab125938 at 1:20 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)
    Rabbit IgG, monoclonal [SP137] - isotype control (ab125938)

参考文献 (8)

ab125938 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab125938 は 8 報の論文で使用されています。

  • Dittfeld C  et al. Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells. PLoS One 13:e0208774 (2018). PubMed: 30532256
  • Ring KL  et al. Potential immunotherapy targets in recurrent cervical cancer. Gynecol Oncol N/A:N/A (2017). IHC . PubMed: 28233576
  • Ceeraz S  et al. VISTA deficiency attenuates antibody-induced arthritis and alters macrophage gene expression in response to simulated immune complexes. Arthritis Res Ther 19:270 (2017). IHC-P ; Human . PubMed: 29216931
  • Dinh PC  et al. Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies. Respir Res 18:132 (2017). Flow Cyt . PubMed: 28666430
  • Wagner KD  et al. Inducible Conditional Vascular-Specific Overexpression of Peroxisome Proliferator-Activated Receptor Beta/Delta Leads to Rapid Cardiac Hypertrophy. PPAR Res 2016:7631085 (2016). IHC-P . PubMed: 27057154
  • Cano E  et al. Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections. Proc Natl Acad Sci U S A 113:656-61 (2016). IHC-P . PubMed: 26739565
  • Long AJ  et al. Syk Inhibition Induces Platelet Dependent Peri-islet Hemorrhage in the Rat Pancreas. Toxicol Pathol 44:998-1012 (2016). PubMed: 27324990
  • Yang Y  et al. Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion. J Perinatol N/A:N/A (2014). PubMed: 25078863

レビューと Q&A

Q&A
質問を送る

1-10 of 13 Q&A

Question

Could you recommend an isotype control antibody for neuropilin 1? See attachment.

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Answer



We can recommend ab125938 (Rabbit IgG, monoclonal [SP137] - isotype control) as isotype control for rabbit monoclonal primary antibodies.

https://www.abcam.com/index.html?datasheet=125938 (or use the following: https://www.abcam.com/index.html?datasheet=125938).

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Question

On the data sheet of the isotype : ab 125938 they mencioned to do antigen retrievel ( AR) with EDTA.

Does that mean that we can’t use this isotype with an antibody who needs AR with citrate ?

When we dilute te isotype 1/100 as they say on the datasheet we have a lot of background !

Kind regards.

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Answer

Thank you for contacting us.

Please note that the Isotype control should be used along with primary antibodies and the concentration of use should match the primary antibody.

The recommended 1/100 dilution is for guidelines only, customer have to optimize the conditions themselves. Regarding the antigen retrieval, it means, this can only be used with antibodies that requires antigen retrieval step.

The isotype control should not give you any staining so if it is showing high background then protocol optimization might be needed e.g. more diluted antibody or optimization of antigen retrieval solution.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Our customer is looking for the isotype control for ab52968 for FACS, ICC/IF and some inhibitory assay. As it was indicated in the reference article of ab52968, also the primary ab ab52968 and its isotype control were purchased from Abcam:



Carr AJ et al. Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay. Mol Vis 15:283-95 (2009). WB.



p.285 To examine the role of MERTK in phagocytosis, we incubated HESC-RPE cells with rabbit monoclonal antibody to 1:40 MERTK or 1:40 control rabbit IgG (both Abcam, Cambridge, UK) for 1 h.



Kindly advise the catalog no. of this isotype control.



Thanks in advance.

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Answer

Unfortunately, in few cases we cannot find the connection between the antibody used in a publication and the antibody used in our catalog, especially as we are trying to link this kind of information to the datasheets.
However, as we have only two matching isotypes in this case, and both differ only in the amount we sell:
https://www.abcam.com/index.html?datasheet=125938 https://www.abcam.com/index.html?datasheet=125938.
https://www.abcam.com/index.html?datasheet=128142 https://www.abcam.com/index.html?datasheet=128142.

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Question

Is the isotype subtype known?

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Answer



I've confirmed with the lab that there is no isotype subclass for rabbit IgG, unlike other species. So the isotype of ab125938 is just IgG.

Here is a reference that mentions the single rabbit isotype-

http://www.pnas.org/content/79/19/6018.full.pdf

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Question

Wir würden gerne wie telefonisch vereinbart die beiden Abs ab92546 Anti-Cardiac Troponin T antibody [EPR3696] und ab125938 Rabbit IgG, monoclonal [SP137] - isotype control für Flow testen.

Bitte senden Sie mir baldmöglichst die Codes für eine rasche Bestellung!

Vielen Dank für Ihre Hilfe,

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Answer

DISCOUNT CODE: ABTRIAL-TBSWY; ABTRIAL-TBSWC

Ablaufdatum: 2013-03-05

Wert: 370€; 360€


Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab92546 und ab125938 in Flow zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Die Discountcodes haben die Nummer ABTRIAL-TBSWY(ab92546) und ABTRIAL-TBSWC (ab125938), deckt den Wert von ab92546 bzw.ab125938 ab und können zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Flow eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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Question

Will these abs work in FLOW?

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Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurden weder ab92546 Anti-Cardiac Troponin T antibody [EPR3696] noch ab125938 Rabbit IgG, monoclonal [SP137] - isotype control bisher in Flow getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab92546 und ab125938 abdecken, und gegen eine erneute Bestellung eines weiteren Produktes von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab92546 und ab125938 in Flow testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie die Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie die Antikörper in Flow.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und wird dann bei der folgenden neuen Bestellung gegen den Preis von ab92546 und ab125938 verrechnet. Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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Question

I realized I forgot to mention the lot#. This is GR83749-1

Could you also tell me the concentration of antibody MMP-14, catalog# ab51074 with lot# GR87560-1.

Thanks again,

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Answer

Thank you for your enquiry.

I can confirm that ab51074 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Today I recieved isotype control ab125938. Could you tell me what the concentration is of this isotype control. I can’t find it on the tube or datasheet.

Thank you.

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Answer

Thank you for contacting us.

The concentration of lot GR83749-1 is XXXmg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Please recommend an isotype control for rabbit IgG.

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Answer

Thank you for contacting us.

The concentration of the current lot of the rabbit monoclonal isotype control we discussed, ab125938, is 0.527 mg/ml. The concentration of the Nrf2 antibody is 0.2 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

is looking for a control for Mouse IgG2b and Rabbit Igg1. He mentioned ab125938 & ab128142, and is asking what is
the difference between these two? Besides the price and the amount, I could not find anything different. He was asking if these
two are "whole", but I don't see that listed on the datasheet. He also mentioned ab88524.

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Answer

My name is Jeremy Kasanov and Tania passed your enquiry to me. She mentions you are looking for a control for Mouse IgG2b and Rabbit Igg1. I am happy to help you find isotype controls for these antibodies. I just need to know:

1.Are your IgG2b and IgG1 antibodies unconjugated, and if sowhat they are conjugated to?

2. What is the concentration and storage buffer of the 2 primary antibodies.

Thanks in advance for the additional information.

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1-10 of 13 Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com