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リコンビナントRabMAb

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

  • Datasheet
  • SDS
Submit a question References (300)

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CUT&RUN - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free FITC HRP PE PerCP

こちらの製品もご検討ください

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ChIC/CUT&RUN pAG-MNase (ab285373)

関連製品

製品の概要

  • 製品名

    Rabbit IgG, monoclonal [EPR25A] - Isotype Control
  • アプリケーション

    適用あり: ICC/IF, Flow Cyt, IHC-P, ChIP-sequencing, IP, ChIC/CUT&RUN-seqmore details
  • 免疫原

    Chemical/ Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

  • 特記事項

    KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR25A
  • アイソタイプ

    IgG

関連製品

  • Alternative Versions

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control  (ab199093)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)
    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
    • Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208150)
    • Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)
    • Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)
    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
    • Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
    • PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab222107)
    • FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)
    • Alexa Fluor® 647 Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab225128)
    • APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
    • ChIP Kit (ab500)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab172730の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Flow Cyt
Use at an assay dependent concentration.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

ChIP-sequencing
Use at an assay dependent concentration. PubMed: 26455392
IP
Use at an assay dependent concentration.
ChIC/CUT&RUN-seq
Use 0.5-2µg for 105 cells.
特記事項
ICC/IF
Use at an assay dependent concentration.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Flow Cyt
Use at an assay dependent concentration.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

ChIP-sequencing
Use at an assay dependent concentration. PubMed: 26455392
IP
Use at an assay dependent concentration.
ChIC/CUT&RUN-seq
Use 0.5-2µg for 105 cells.

画像

  • CUT&RUN - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    CUT&RUN - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ab285373 105 HeLa cells and 0.5 µg, 1 µg  and 2µg of ab172730 [EPR25A]. H3K4me3 (ab213224) and CTCF (ab188408) are used for comparison. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads

    Additional screenshots of mapped reads can be downloaded here.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Vimentin RabMAb (ab92547, left panel) (brown) and Rabbit mAb IgG control (ab172730, right panel).

  • Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    LINE-1 ORF1p was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate with ab216324 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216324 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: F9 whole cell lysate 10 µg (Input). 
    Lane 2: ab216324 IP in F9 whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216324 in F9 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    ab124962 (purified) at 1/20 immunoprecipitating IL-1RA in NIH/3T3 whole cell lysate.
    Lane 1 (input): NIH/3T3 whole cell lysate (10µg)
    Lane 2 (+): ab124962 + NIH/3T3 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124962 in NIH/3T3 whole cell lysate.
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Overlay histogram showing A549 (human lung carcinoma) cells stained with ab133557 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab133557 at 1/60 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.

    Conjugated versions are available for this clone: Alexa Fluor® 488 (ab199091), Alexa Fluor® 647 (ab199093), R-PE (ab209478), APC (ab232814).

  • Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Overlay histogram showing K562 (human chronic myelogenous leukemia) cells stained with ab196018 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab196018 at 1/150 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Vimentin RabMAb (ab92547, left panel) (brown) and Rabbit mAb IgG control (ab172730, right panel).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue with unpurified Rabbit IgG ab172730 at 1/10. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue with purified Rabbit IgG ab172730 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Overlay histogram showing SH-SY5Y (human neuroblastoma) cells stained with ab179513 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab179513 at 1/150 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.

  • Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Overlay histogram showing A549 (human lung carcinoma) cells stained with ab185633 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab185633 at 1/150 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Immunofluorescent staining of HeLa cells using anti-AIF RabMAb (ab32516, left panel) (green) and Rabbit mAb IgG control (ab172730, right panel). DAPI nuclear staining (blue).

    Conjugated versions are available for this clone: Alexa Fluor® 488 (ab199091), Alexa Fluor® 647 (ab199093), R-PE (ab209478), APC (ab232814).

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Immunocytochemistry/immunofluorescence analysis of HeLa cells with purified Rabbit IgG ab172730 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Immunocytochemistry/immunofluorescence analysis of HeLa cells with unpurified Rabbit IgG ab172730 at 1/10. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (300)

ab172730 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab172730 は 300 報の論文で使用されています。

  • Radhakrishnan R & Kowluru RA Long Noncoding RNA MALAT1 and Regulation of the Antioxidant Defense System in Diabetic Retinopathy. Diabetes 70:227-239 (2021). PubMed: 33051272
  • Chang L  et al. LncRNA RP11-84E24.3 drives tumorigenesis and epithelial-to-mesenchymal transition of glioma cells by promoting TFAP2C-mediated activation of SNAI1. J Neurooncol 151:157-171 (2021). PubMed: 33128099
  • Yao W  et al. TNK2-AS1 upregulated by YY1 boosts the course of osteosarcoma through targeting miR-4319/WDR1. Cancer Sci 112:893-905 (2021). PubMed: 33164271
  • Fang J  et al. Investigating the effect of lncRNA HOTAIR on apoptosis induced by myocardial ischemia-reperfusion injury. Mol Med Rep 23:N/A (2021). PubMed: 33398378
  • Zhang A  et al. TNK2 promoted esophageal cancer progression via activating egfr-akt signaling. J Clin Lab Anal 35:e23700 (2021). PubMed: 33484472
View all Publications for this product

レビューと Q&A

Q&A
質問を送る

There are currently no Questions for ab172730.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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