Rabbit Anti-Human IgG+IgM+IgA H&L (FITC) (ab8512)

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Key features and details

  • Rabbit Anti-Human IgG+IgM+IgA H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Rabbit
  • Suitable for: ICC/IF

製品の概要

  • 製品名

    Rabbit Anti-Human IgG+IgM+IgA H&L (FITC)
    IgG + IgM + IgA 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Human

  • 特異性

    Antibodies were raised in rabbit to human IgG, IgM and IgA

  • アプリケーション

    適用あり: ICC/IFmore details

  • 標識

    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.3
    Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Specific antibodies were absorpted by incubation with Sepharose-bound human immuno-globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human immuno-globulins a minimum of 65% protein bound.

  • 特記事項（標識）

    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.

  • ポリ/モノ

    ポリクローナル

  • 特記事項

    F/P ratio is 4-6 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where Ig's are depostited.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab8512 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8512 は 1 報の論文で使用されています。

  • Pisa D  et al. Evolution of antibody response and fungal antigens in the serum of a patient infected with Candida famata. J Med Microbiol 56:571-8 (2007). ICC/IF . PubMed: 17446276

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