Rabbit Anti-Human IgG H&L (HRP) (ab6759)

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ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)

    Key features and details

    • Rabbit Anti-Human IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Rabbit
    • Isotype: IgG
    • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA

    製品の概要

    • 製品名

      Rabbit Anti-Human IgG H&L (HRP)
      IgG 二次抗体 製品一覧

    • 由来種

      Rabbit

    • ターゲット生物種

      Human

    • アプリケーション

      適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details

    • 免疫原

      human IgG whole molecule

    • 標識

      HRP

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 精製度

      Affinity purified

    • 特記事項（精製）

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 特記事項（標識）

      Horseradish Peroxidase (HRP)

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6759の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Dot blot
    Use at an assay dependent dilution.
    ELISA (1)
    1/130000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/5000 - 1/25000.
    ICC/IF
    1/1000 - 1/5000.
    Competitive ELISA
    Use at an assay dependent dilution. PubMed: 20573830
    特記事項
    Dot blot
    Use at an assay dependent dilution.
    ELISA
    1/130000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/5000 - 1/25000.
    ICC/IF
    1/1000 - 1/5000.
    Competitive ELISA
    Use at an assay dependent dilution. PubMed: 20573830

    画像

    • ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)
      ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)

      ELISA results of ab6759 tested against purified human IgG protein.

      Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (49)

    ab6759 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6759 は 49 報の論文で使用されています。

    • Zhu LJ  et al. BUB1 promotes proliferation of liver cancer cells by activating SMAD2 phosphorylation. Oncol Lett 19:3506-3512 (2020). PubMed: 32269624
    • Orlowski A  et al. Successful Transduction with AAV Vectors after Selective Depletion of Anti-AAV Antibodies by Immunoadsorption. Mol Ther Methods Clin Dev 16:192-203 (2020). PubMed: 32055647
    • Yuan YF  et al. Cyclopamine functions as a suppressor of benign prostatic hyperplasia by inhibiting epithelial and stromal cell proliferation via suppression of the Hedgehog signaling pathway. Int J Mol Med 46:311-319 (2020). PubMed: 32319534
    • Wang X  et al. Adenovirus delivery of encoded monoclonal antibody protects against different types of influenza virus infection. NPJ Vaccines 5:57 (2020). PubMed: 32665862
    • Eick GN  et al. Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots. J Physiol Anthropol 39:16 (2020). PubMed: 32678000
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Intracellular Alpha-galactosidase A/antibody-complex detection from enzyme-treated EA.hy926 cells

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Cells were seeded on 96-well plates with a density of 2x105 cell/ml and grown until confluent. After 4 hours of incubation with human recombinant α-galactosidase A (AGAL, 4.6 μg/ml) and patients’ serum (50 µl per 4.6 μg/ml AGAL), cells were washed twice with PBS and lysed with passive lysis buffer (Promega, Mannheim Germany). Lysates were centrifuged (14.000x g) for 20 minutes at 4°C and supernatants were used for ELISAs, as follows. 96 well plates were coated with a capture antibody (α-AGAL, R&D Sytems, Minneapolis, US no. AF6146, 200 ng per well) over night at 4°C. After 5 washes with 200 μl PBS/Tween 0.1% and blocking with PBS/BSA 2% for 2 hours at RT, 3-10 μl of cellular lysates were applied in 100 μl PBS overnight at 4°C. Wells were washed 3 times with PBS/Tween 0.1%. For human IgG detection shuttled into cells by AGAL, anti-hIgG HRP-coupled antibodies (Abcam no: ab6759, working concentration: 0.66 μg/ml) were applied in PBS/BSA 2% for 2 hours at RT. After 5 additional washes, 100 μl 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added to each well, incubated at RT, and stopped with 100 μl 2 mol/l sulfuric acid. Absorption was measured at 450 nm and normalized for protein content and against serum from a healthy control (negativ for antibodies against AGAL).

    Figure legend: *p<0.05, **p<0.01.

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    投稿 Sep 04 2019

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