Rabbit Anti-Human IgG H&L (HRP) (ab6759)
Key features and details
- Rabbit Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA
製品の概要
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製品名
Rabbit Anti-Human IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Human -
アプリケーション
適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details -
免疫原
human IgG whole molecule
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Horseradish Peroxidase (HRP) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
Our Abpromise guarantee covers the use of ab6759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Dot blot | Use at an assay dependent dilution. | |
ELISA | 1/130000. | |
IHC-P | Use at an assay dependent dilution. | |
IHC-Fr | Use at an assay dependent dilution. | |
Immunomicroscopy | Use at an assay dependent dilution. | |
WB | 1/5000 - 1/25000. | |
ICC/IF | 1/1000 - 1/5000. | |
Competitive ELISA | Use at an assay dependent dilution. PubMed: 20573830 |
画像
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ELISA results of ab6759 tested against purified human IgG protein.
Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (42)
ab6759 は 42 報の論文で使用されています。
- Zhu LJ et al. BUB1 promotes proliferation of liver cancer cells by activating SMAD2 phosphorylation. Oncol Lett 19:3506-3512 (2020). PubMed: 32269624
- Xu J et al. Knockdown of serpin peptidase inhibitor clade C member 1 inhibits the growth of nasopharyngeal carcinoma cells. Mol Med Rep 19:3658-3666 (2019). PubMed: 30896875
- Yang Y et al. Clinical Features and Cytokine Profile in Myositis Patients with Anti-EJ Autoantibodies Detected by a Novel Immunoprecipitation Assay. Biomed Res Int 2019:1856180 (2019). PubMed: 31019965
- Ma TZ et al. Differential expression of serum proteins in multiple myeloma. Exp Ther Med 17:649-656 (2019). PubMed: 30651846
- Yuan G et al. Tumor suppressor role of microRNA-545 in oral squamous cell carcinoma. Oncol Lett 17:2063-2068 (2019). PubMed: 30675273