Rabbit Anti-Human IgG H&L (HRP) (ab6759)
Key features and details
- Rabbit Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA
Related conjugates and formulations
製品の概要
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製品名
Rabbit Anti-Human IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Human -
アプリケーション
適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details -
免疫原
human IgG whole molecule
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Horseradish Peroxidase (HRP) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6759の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
Use at an assay dependent dilution.
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ELISA | (1) |
1/130000.
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IHC-P |
Use at an assay dependent dilution.
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|
IHC-Fr |
Use at an assay dependent dilution.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
WB |
1/5000 - 1/25000.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
Competitive ELISA |
Use at an assay dependent dilution. PubMed: 20573830
|
特記事項 |
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Dot blot
Use at an assay dependent dilution. |
ELISA
1/130000. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
WB
1/5000 - 1/25000. |
ICC/IF
1/1000 - 1/5000. |
Competitive ELISA
Use at an assay dependent dilution. PubMed: 20573830 |
画像
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ELISA results of ab6759 tested against purified human IgG protein.
Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (115)
ab6759 は 115 報の論文で使用されています。
- Zhang X et al. Altered genome‑wide hydroxymethylation analysis for neoadjuvant chemoradiotherapy followed by surgery in esophageal cancer. Exp Ther Med 25:29 (2023). PubMed: 36561617
- Umemoto A et al. Identification of anti-citrullinated osteopontin antibodies and increased inflammatory response by enhancement of osteopontin binding to fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther 25:25 (2023). PubMed: 36804906
- Zeng Q et al. TRIM59 attenuates ox-LDL-induced endothelial cell inflammation, apoptosis, and monocyte adhesion through AnxA2. Ann Transl Med 11:42 (2023). PubMed: 36819529
- Martin-Souto L et al. Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients. J Fungi (Basel) 9:N/A (2023). PubMed: 36836272
- Yang J et al. KIF18A interacts with PPP1CA to promote the malignant development of glioblastoma. Exp Ther Med 25:154 (2023). PubMed: 36911368