Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (ab6760)

Submit a review Q&A (1)References (4)

Key features and details

  • Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISA

製品の概要

  • 製品名

    Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Human

  • アプリケーション

    適用あり: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISAmore details

  • 免疫原

    human IgG whole molecule

  • 標識

    Alkaline Phosphatase

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 50% Glycerol
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads.

  • 特記事項（標識）

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunomicroscopy
WB
ICC/IF
IHC-Fr
Dot blot
IHC-P
ELISA
  • 追加情報
    suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency

    • プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (4)

    ab6760 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6760 は 4 報の論文で使用されています。

    • Royle KE & Polizzi K A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. Sci Rep 7:15817 (2017). WB . PubMed: 29150665
    • Iversen R  et al. Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells. J Immunol 190:5981-91 (2013). PubMed: 23690478
    • Kap YS  et al. Fast Progression of Recombinant Human Myelin/Oligodendrocyte Glycoprotein (MOG)-Induced Experimental Autoimmune Encephalomyelitis in Marmosets Is Associated with the Activation of MOG34-56-Specific Cytotoxic T Cells. J Immunol 180:1326-37 (2008). ELISA . PubMed: 18209026
    • Jagessar SA  et al. Autoimmunity Against Myelin Oligodendrocyte Glycoprotein Is Dispensable for the Initiation Although Essential for the Progression of Chronic Encephalomyelitis in Common Marmosets. J Neuropathol Exp Neurol 67:326-340 (2008). ELISA . PubMed: 18379435

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    does this antibody recognize human kappa light chains? lambda? both?

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    Answer

    ab6760 is produced against polyclonal whole human IgG purified from serum, and the kappa:lambda ratio of IgG normal human serum is reported to be ~2:1. ab6760 is not tested specifically against kappa or lambda light chains, but it is expected to recognize both forms as they are normally present in human serum.

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