Key features and details
- Rabbit Anti-Human IgA H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Suitable for: Dot blot, ELISA
保存方法Shipped at 4°C. Store at -20°C.
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
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精製度Immunogen affinity purified
特記事項（精製）From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
特記事項（標識）The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
Our Abpromise guarantee covers the use of ab8510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab8510 は 2 報の論文で使用されています。
- Kempsell KE et al. Whole genome protein microarrays for serum profiling of immunodominant antigens of Bacillus anthracis. Front Microbiol 6:747 (2015). ELISA . PubMed: 26322022
- Csorba K et al. Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases. Orphanet J Rare Dis 6:31 (2011). ELISA . PubMed: 21619684