Key features and details
- Rabbit Anti-Human IgA H&L
- Host species: Rabbit
- Suitable for: ELISA, Dot blot
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride
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精製度Immunogen affinity purified
特記事項（精製）From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgG and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
Our Abpromise guarantee covers the use of ab8508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recommended dilution as secondary antibody in dot-blotting is 1:100.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.