Rabbit Anti-Goat IgG H&L (HRP) preadsorbed (ab97105)
Key features and details
- Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
製品の概要
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製品名
Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Goat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
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アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
吸着処理血清
Chicken, Horse, Human, Mouse, Pig, Rat more details -
免疫原
Other Immunogen Type corresponding to Goat IgG.
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97105の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. ELISA-Primary
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WB | (1) |
1/5000 - 1/50000. WB-Colorimetric 1:5,000 - 1:30,000; WB-Chemiluminescent 1:10,000 - 1:50,000
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特記事項 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. ELISA-Primary |
WB
1/5000 - 1/50000. WB-Colorimetric 1:5,000 - 1:30,000; WB-Chemiluminescent 1:10,000 - 1:50,000 |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab97105 は 10 報の論文で使用されています。
- Reithmair M et al. Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance. Methods Mol Biol 2504:113-133 (2022). PubMed: 35467283
- Kirchner B et al. Postprandial transfer of colostral extracellular vesicles and their protein and miRNA cargo in neonatal calves. PLoS One 15:e0229606 (2020). PubMed: 32109241
- Buschmann D et al. Propofol and Sevoflurane Differentially Impact MicroRNAs in Circulating Extracellular Vesicles during Colorectal Cancer Resection: A Pilot Study. Anesthesiology 132:107-120 (2020). PubMed: 31596735
- Mussack V et al. Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research. Biomol Detect Quantif 17:100089 (2019). PubMed: 31194192
- Madan E et al. HIF-transcribed p53 chaperones HIF-1a. Nucleic Acids Res 47:10212-10234 (2019). PubMed: 31538203
- Hermann S et al. Transcriptomic profiling of cell-free and vesicular microRNAs from matched arterial and venous sera. J Extracell Vesicles 8:1670935 (2019). PubMed: 31632620
- Buschmann D et al. Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing. J Extracell Vesicles 7:1481321 (2018). PubMed: 29887978
- Chen T et al. Circ_0078767 suppresses non-small-cell lung cancer by protecting RASSF1A expression via sponging miR-330-3p. Cell Prolif N/A:e12548 (2018). PubMed: 30507050
- Cimica V et al. Novel Respiratory Syncytial Virus-Like Particle Vaccine Composed of the Postfusion and Prefusion Conformations of the F Glycoprotein. Clin Vaccine Immunol 23:451-9 (2016). PubMed: 27030590
- Ogawa S et al. Shotgun proteomic analysis of porcine colostrum and mature milk. Anim Sci J 85:440-8 (2014). PubMed: 24450292