Rabbit Anti-Goat IgG H&L (HRP) preadsorbed (ab7132)

Submit a review Q&A (4)References (4)

Key features and details

  • Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICC

製品の概要

  • 製品名

    Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Goat

  • 特異性

    Pre-adsorbed against Human Serum Proteins to minimise reactivity.

  • アプリケーション

    適用あり: ELISA, IHC-P, IHC-Fr, WB, ICCmore details

  • 吸着処理血清


    Human more details

  • 免疫原

    Goat IgG whole molecule

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.60
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Horseradish Peroxidase (HRP)

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000 - 1/120000.
IHC-P 1/500 - 1/2500.
IHC-Fr 1/500 - 1/2500.
WB 1/1000 - 1/5000.
ICC 1/500 - 1/2500.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (4)

ab7132 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7132 は 4 報の論文で使用されています。

  • Rundqvist HC  et al. Influence of nutrient ingestion on amino acid transporters and protein synthesis in human skeletal muscle after sprint exercise. J Appl Physiol (1985) 123:1501-1515 (2017). PubMed: 28860165
  • Moberg M  et al. Activation of mTORC1 by leucine is potentiated by branched-chain amino acids and even more so by essential amino acids following resistance exercise. Am J Physiol Cell Physiol 310:C874-84 (2016). PubMed: 27053525
  • Apró W  et al. Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling. Am J Physiol Endocrinol Metab 308:E470-81 (2015). PubMed: 25605643
  • Kassner N  et al. CBR1 is a predominant doxorubicin reductase in the human liver. Drug Metab Dispos : (2008). WB . PubMed: 18635746

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

What is best secondary antibody you can offer for ab10256.

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Answer

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Dear Sir/Madam,



I am trying to order an antibody from your website but i can’t seem to find the product anywhere on-line, perhaps it has been discontinued or is out of stock?



The product # is 92281 a MAFbx primary antibody. I also needed the appropriate secondary goat antibody (# 7132) which is also unavailable. Can you suggest the next best possible alternatives please? Or let me know if these antibodies are likely to be in stock sometime soon?



Many thanks

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Answer

Thank you for your enquiry and your interest in our products.



I can confirm that ab92281 is Anti-Fbx32 antibody and ab7132 is Rabbit anti-Goat IgG H&L (HRP) secondary antibody; and both products are still published and available.



ab92281: https://www.abcam.com/fbx32-antibody-ab92281.html

ab7132: https://www.abcam.com/rabbit-polyclonal-secondary-antibody-to-goat-igg-h-l-hrp-pre-adsorbed-ab7132.html



If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Hi,   What secondary HRP-linked antibody would you recommend after incubation with ab92281 (FBX32 ab)?

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Answer

Thank you for your enquiry. I can recommend the following HRP-conjugated secondary antibody based on your requirements: Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed (ab7132) I hope this is helpful. Please contact us again if you have any further questions.

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Question

I have sheep polyclonal to 5-methyl cytosine antibody (ab1884) as first antibody, and Rabbit polyclonal to goat IgG H&L (HRP) (ab7132) as second antibody. Dose rabbit IgG to goat react with sheep IgG?

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Answer

Thank you for your enquiry. I have been in touch with the lab and determined that this antibody has NOT been preabsorbed against sheep IgG. I am sorry that I cannot be of more assistance on this occasion.

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