Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) (ab169349)

製品の概要

  • 製品名

    Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647)
    IgG 二次抗体 製品一覧
  • 製品の詳細

    Rabbit F(ab')2 polyclonal Secondary Antibody to Rat (Alexa Fluor® 647)
  • 由来種

    Rabbit
  • ターゲット生物種

    Rat
  • 特異性

    This antibody is specific to Rat IgG
  • アプリケーション

    適用あり: IHC-Fr, Flow Cyt, ICC/IF, ELISA, IHC-Pmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • ポリ/モノ

    ポリクローナル
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab169349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/2000.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

画像

  • ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1µg/ml) overnight at +4°C. Ab169349, Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) (red) was then used at 1µg/ml for 1h at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab169349, Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) (red) was used at 1µg/ml for 1h at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab169349 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
The spectral location in the red made this perfect for a double label with the other fluorophore in the blue. It was easy to use and penetrated well after Tween 80 treatment. It has stood up well to storage in the refrigerator and gives a good signal after 6 months.

Prof. Martin Mendelson

Verified customer

投稿 Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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