Key features and details
- Rabbit F(ab')2 Anti-Mouse IgG - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA, IHC-P
製品名Rabbit F(ab')2 Anti-Mouse IgG - H&L (Biotin), pre-adsorbed
IgG 二次抗体 製品一覧
特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
アプリケーション適用あり: WB, ELISA, IHC-Pmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Mouse IgG whole molecule
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
特記事項（精製）This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
特記事項（標識）Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG F(ab')2 molecule
Our Abpromise guarantee covers the use of ab5761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|ELISA||1/20000 - 1/100000.|
|IHC-P||1/1000 - 1/5000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.