Rabbit F(ab')2 Anti-Goat IgG - H&L (HRP), pre-adsorbed (ab5755)

Submit a review Q&A (1)References (2)

Key features and details

  • Rabbit F(ab')2 Anti-Goat IgG - H&L (HRP), pre-adsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC

製品の概要

  • 製品名

    Rabbit F(ab')2 Anti-Goat IgG - H&L (HRP), pre-adsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Goat

  • 特異性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.

  • アプリケーション

    適用あり: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details

  • 吸着処理血清


    Human more details

  • 免疫原

    Goat IgG whole molecule.

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 特記事項（標識）

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/10000.
ELISA 1/10000 - 1/50000. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab5755 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5755 は 2 報の論文で使用されています。

  • Díaz-Jiménez D  et al. A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis. Sci Rep 7:10180 (2017). PubMed: 28860510
  • Belian E  et al. Forward Programming of Cardiac Stem Cells by Homogeneous Transduction with MYOCD plus TBX5. PLoS One 10:e0125384 (2015). WB . PubMed: 26047103

レビューと Q&A

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Question

What is best secondary antibody you can offer for ab10256.

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Answer

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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