Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed (ab5753)
Key features and details
- Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC, Immunomicroscopy, Flow Cyt
製品の概要
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製品名
Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Goat -
特異性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins. -
アプリケーション
適用あり: WB, ICC/IF, Dot blot, ELISA, IHC, Immunomicroscopy, Flow Cytmore details -
吸着処理血清
Human more details -
免疫原
Goat IgG whole molecule
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5753の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
Dot blot |
1/10000.
|
|
ELISA |
1/20000 - 1/100000.
|
|
IHC |
1/500 - 1/5000.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
Flow Cyt |
1/200 - 1/1000.
|
特記事項 |
---|
WB
1/2000 - 1/10000. |
ICC/IF
1/1000 - 1/5000. |
Dot blot
1/10000. |
ELISA
1/20000 - 1/100000. |
IHC
1/500 - 1/5000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
1/200 - 1/1000. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab5753 は 1 報の論文で使用されています。
- Chamberlain G et al. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS ONE 3:e2934 (2008). Flow Cyt . PubMed: 18698345