Key features and details
- Rabbit F(ab')2 polyclonal Secondary Antibody to Goat (Alexa Fluor® 647)
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Rabbit
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA
製品名Rabbit F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 647)
IgG 二次抗体 製品一覧
製品の詳細Rabbit F(ab')2 polyclonal Secondary Antibody to Goat (Alexa Fluor® 647)
特異性This antibody is specific to Goat IgG
アプリケーション適用あり: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
The details of the immunogen for this antibody are not available.
標識Alexa Fluor® 647. Ex: 652nm, Em: 668nm
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
バッファーPreservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Our Abpromise guarantee covers the use of ab169347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||1/200 - 1/1000.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab169347, Rabbit F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 647) (red) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab169347, Rabbit F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 647) (red) was used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.