Rabbit Anti-Chicken IgY H&L (HRP) (ab97140)
Key features and details
- Rabbit Anti-Chicken IgY H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
製品の概要
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製品名
Rabbit Anti-Chicken IgY H&L (HRP)
IgY 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Chicken -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項(標識)
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Part of the AbExcel range. -
研究分野
関連製品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97140の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. (Primary).
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WB |
1/2000 - 1/20000. Colorimetric: Use 1/5,000-1/30,000, Chemiluminescent: Use 1/10,000-1/50,000
|
特記事項 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. (Primary). |
WB
1/2000 - 1/20000. Colorimetric: Use 1/5,000-1/30,000, Chemiluminescent: Use 1/10,000-1/50,000 |
画像
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All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/2000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/10000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/20000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab97140 は 7 報の論文で使用されています。
- Sáenz L et al. Efficacy of Multivalent, Cochleate-Based Vaccine against Salmonella Infantis, S. Enteritidis and S. Typhimurium in Laying Hens. Vaccines (Basel) 10:N/A (2022). PubMed: 35214684
- Hu R et al. An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in Poultry. Nanomaterials (Basel) 10:N/A (2020). PubMed: 33233490
- Nery-Flores SD et al. Dietary Curcumin Prevented Astrocytosis, Microgliosis, and Apoptosis Caused by Acute and Chronic Exposure to Ozone. Molecules 24:N/A (2019). PubMed: 31387223
- Wolfes AC et al. A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes. J Gen Physiol 149:149-170 (2017). PubMed: 27908976
- Nakao LS et al. Mechanism-based proteomic screening identifies targets of thioredoxin-like proteins. J Biol Chem 290:5685-95 (2015). PubMed: 25561728
- Thiede-Stan NK et al. Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex. J Cell Sci 128:3583-96 (2015). PubMed: 26290381
- Rainczuk A et al. Evidence for the antagonistic form of CXC-motif chemokine CXCL10 in serous epithelial ovarian tumours. Int J Cancer 134:530-41 (2014). PubMed: 23873303