Rabbit Anti-Chicken IgY H&L (FITC) (ab6749)

Submit a review Q&A (2)References (9)

Key features and details

  • Rabbit Anti-Chicken IgY H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA

製品の概要

  • 製品名

    Rabbit Anti-Chicken IgY H&L (FITC)
    IgY 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Chicken

  • アプリケーション

    適用あり: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details

  • 免疫原

    chicken IgY whole molecule

  • 標識

    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6749の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000. PubMed: 18983843
ELISA
1/10000 - 1/50000.
特記事項
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000. PubMed: 18983843
ELISA
1/10000 - 1/50000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (9)

ab6749 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6749 は 9 報の論文で使用されています。

  • Kiese K  et al. Epigenetic control of epilepsy target genes contributes to a cellular memory of epileptogenesis in cultured rat hippocampal neurons. Acta Neuropathol Commun 5:79 (2017). PubMed: 29089052
  • Cheng YC  et al. Albumin overload down-regulates integrin-ß1 through reactive oxygen species-endoplasmic reticulum stress pathway in podocytes. J Biochem 158:101-8 (2015). PubMed: 25713411
  • Buijs PR  et al. Different responses of human pancreatic adenocarcinoma cell lines to oncolytic Newcastle disease virus infection. Cancer Gene Ther 21:24-30 (2014). PubMed: 24384773
  • Na GH  et al. Effects of glucose concentration in the medium on rat hepatocyte culture. Ann Surg Treat Res 87:53-60 (2014). ICC/IF . PubMed: 25114883
  • Boulanger CA  et al. Embryonic stem cells are redirected to non-tumorigenic epithelial cell fate by interaction with the mammary microenvironment. PLoS One 8:e62019 (2013). IHC-P ; Mouse . PubMed: 23637952
  • Higgins S  et al. Inducing neurite outgrowth by mechanical cell stretch. Biores Open Access 2:212-6 (2013). ICC/IF . PubMed: 23741633
  • Kuo YC  et al. Capillary electrophoresis of bone marrow stromal cells with uptake of heparin-functionalized poly(lactide-co-glycolide) nanoparticles during differentiation towards neurons. Electrophoresis 31:315-23 (2010). ICC/IF . PubMed: 20084630
  • Tomasi ML  et al. S-adenosylmethionine regulates apurinic/apyrimidinic endonuclease 1 stability: implication in hepatocarcinogenesis. Gastroenterology 136:1025-36 (2009). ICC/IF . PubMed: 18983843
  • Papalouka V  et al. Muscle LIM protein interacts with cofilin 2 and regulates F-actin dynamics in cardiac and skeletal muscle. Mol Cell Biol 29:6046-58 (2009). ICC/IF . PubMed: 19752190

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1-2 of 2 Abreviews or Q&A

Question

I want to label the antiserum of chicken, which product I should chose for my Flowcytomry analysis (specifically FITC) among ab6749,ab7114 and ab6873? I noticed that ab7114's immunogen is full length native chicken IgY (purified). In addition, whether these there products can be replaced for each other?

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Answer

Thank you for your enquiry. ab6749, ab7114 and ab6873 are all suitable for flow cytometry analysis, have all been raised against chicken IgY and are all FITC conjugated. There is very little difference between the three aside from the mass of antibody sold. ab6749 is sold as 1.5mg, whereas ab7114 is sold as 100 µg and ab6873 as 1 mg. All are sold at the same price £ 98.00/$ 150.00/ € 165.00.

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Question

What protocols have been used for this antibody in immunofluorescence? Blocking, incubation times, dilutions. Any specific reference?

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Answer

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. We do not have a specific protocol for this antibody in immunofluorescence, or a specific reference. If you have any more questions, please contact us again.

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