Rabbit C Reactive Protein (PTX1) ELISA Kit (ab157726)
Key features and details
- Sensitivity: 2.0828 ng/ml
- Range: 6.25 ng/ml - 200 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
製品の概要
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製品名
Rabbit C Reactive Protein (PTX1) ELISA Kit
C Reactive Protein キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall < 10% -
サンプルの種類
Serum, Plasma, Other biological fluids -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.0828 ng/ml -
検出範囲
6.25 ng/ml - 200 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum > 85 % - % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rabbit -
製品の概要
Abcam’s C Reactive Protein (PTX1) Rabbit ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C Reactive Protein levels in rabbit serum and plasma.
In this assay the C Reactive Protein present in samples reacts with the anti-C Reactive Protein antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C Reactive Protein antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C Reactive Protein. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C Reactive Protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C Reactive Protein in the test sample. The quantity of C Reactive Protein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X HRP-conjugated anti-rabbit C Reactive Protein antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rabbit C Reactive Protein Calibrator (lyophilized) 1 vial Rabbit C Reactive Protein ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究分野
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機能
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
組織特異性
Found in plasma. -
配列類似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab157726 は 5 報の論文で使用されています。
- Poilvache H et al. Development of an innovative in vivo model of PJI treated with DAIR. Front Med (Lausanne) 9:984814 (2022). PubMed: 36314026
- Pérez-Baos S et al. Inhibition of pSTAT1 by tofacitinib accounts for the early improvement of experimental chronic synovitis. J Inflamm (Lond) 16:2 (2019). PubMed: 30728752
- Pérez-Baos S et al. Tofacitinib restores the inhibition of reverse cholesterol transport induced by inflammation: understanding the lipid paradox associated with rheumatoid arthritis. Br J Pharmacol 174:3018-3031 (2017). PubMed: 28646516
- Little RD et al. Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. Sci Rep 7:6311 (2017). PubMed: 28740214
- Larrañaga-Vera A et al. Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis. Arthritis Res Ther 19:264 (2017). PubMed: 29191221