Anti-RAB7B 抗体 [EPR15727] (ab193360)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15727] to RAB7B
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RAB7B antibody [EPR15727]
RAB7B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15727] to RAB7B -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- THP-1, 293, U937 and NIH/3T3 cell lysates; Human skeletal muscle and prostatic hyperplasia tissues; U937 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15727 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab193360の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/270.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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IHC-P |
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/270. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway. -
組織特異性
Expressed in heart, placenta, lung, skeletal muscle and peripheral blood leukocyte. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
細胞内局在
Late endosome. Lysosome. Golgi apparatus > trans-Golgi network. Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 338382 Human
- Entrez Gene: 226421 Mouse
- SwissProt: Q96AH8 Human
- SwissProt: Q8VEA8 Mouse
- Unigene: 534612 Human
- Unigene: 44508 Mouse
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別名
- MGC16212 antibody
- MGC9726 antibody
- RAB7 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-RAB7B antibody [EPR15727] (ab193360) at 1/1000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : U937 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-RAB7B antibody [EPR15727] (ab193360) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed U937 cells labeling RAB7B with ab193360 at 1/270 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab193360 は論文での使用が確認できていません。