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    rab5-antibody-early-endosome-marker-ab18211.pdf

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Neuroscience Neurotransmission Secretory Vesicles Rabs
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Anti-Rab5 抗体 - Early Endosome Marker (ab18211)

  • Datasheet
  • SDS
Reviews (26)Q&A (9)References (96)

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Immunocytochemistry - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
  • Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
  • Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
  • Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)

Key features and details

  • Rabbit polyclonal to Rab5 - Early Endosome Marker
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Rab5 antibody [EPR21801] (ab218624)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Rab5 antibody - Early Endosome Marker
    Rab5 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Rab5 - Early Endosome Marker
  • 由来種

    Rabbit
  • 特異性

    The immunogen used for this product shares 43% homology with Rab5C. Cross-reactivity with this protein has not been confirmed experimentally.
  • アプリケーション

    適用あり: WB, ICCmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Human Rab5 aa 150 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab18625)

  • ポジティブ・コントロール

    • ICC: HeLa cells
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Rabs
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Rab5 protein (ab62956)
  • Recombinant Protein

    • Recombinant Human Rab5 protein (ab62956)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18211の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (15)
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC
Use a concentration of 1 µg/ml.
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC
Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Required for the fusion of plasma membranes and early endosomes.
  • 配列類似性

    Belongs to the small GTPase superfamily. Rab family.
  • 細胞内局在

    Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
  • Target information above from: UniProt accession P20339 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 404008 Dog
    • Entrez Gene: 5868 Human
    • Entrez Gene: 271457 Mouse
    • Entrez Gene: 64633 Rat
    • Omim: 179512 Human
    • SwissProt: P18066 Dog
    • SwissProt: P20339 Human
    • SwissProt: Q9CQD1 Mouse
    • SwissProt: M0RC99 Rat
    • Unigene: 475663 Human
    • Unigene: 329123 Mouse
    see all
  • 別名

    • RAB 5 antibody
    • RAB 5A antibody
    • RAB5A antibody
    • RAB5A member RAS oncogene family antibody
    • RAB5A_HUMAN antibody
    • RAS associated protein RAB5A antibody
    • Ras related protein Rab 5A antibody
    • Ras-related protein Rab-5A antibody
    see all

画像

  • Immunocytochemistry - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
    Immunocytochemistry - Anti-Rab5 antibody - Early Endosome Marker (ab18211)

    ab18211 staining Rab5 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab18211 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
    Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
    Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/ml + Jurkat cell lysate at 20 µg

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa
    Additional bands at: 40 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
    Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
    All lanes : Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/ml

    Lane 1 : NIH/3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770)
    Lane 3 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 5 : Spinal Cord (Mouse) Tissue Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 7 : Brain (Rat) Tissue Lysate - normal tissue
    Lane 8 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
    Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
    Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/ml + Recombinant Human Rab5 protein (ab62956) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa


    Exposure time: 150 seconds

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (96)

ab18211 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18211 は 96 報の論文で使用されています。

  • Gautier MK & Ginsberg SD A method for quantification of vesicular compartments within cells using 3D reconstructed confocal z-stacks: Comparison of ImageJ and Imaris to count early endosomes within basal forebrain cholinergic neurons. J Neurosci Methods 350:109038 (2021). PubMed: 33338543
  • Lv J  et al. Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages. Cell Discov 7:24 (2021). PubMed: 33850112
  • Vandendriessche C  et al. Importance of extracellular vesicle secretion at the blood-cerebrospinal fluid interface in the pathogenesis of Alzheimer's disease. Acta Neuropathol Commun 9:143 (2021). PubMed: 34425919
  • Huang X  et al. Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes. ACS Nano 14:13192-13202 (2020). PubMed: 32940450
  • Pensalfini A  et al. Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease. Cell Rep 33:108420 (2020). PubMed: 33238112
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 35 Abreviews or Q&A

Western blot abreview for Anti-Rab5 antibody - Early Endosome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Jun 10 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rab5 antibody - Early Endosome Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain section - 50um)
Antigen retrieval step
None
Permeabilization
Yes - PBS-T 3 x 5mn
Specification
Brain section - 50um
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 13 2019

Western blot abreview for Anti-Rab5 antibody - Early Endosome Marker

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Podocytes)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
75 µg
Specification
Mouse Podocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Apr 30 2018

Immunoprecipitation abreview for Anti-Rab5 antibody - Early Endosome Marker

Average
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Miapaca2)
Total protein in input
1000 µg
Treatment
EGF 50 ng/ml, for 16 h
Immuno-precipitation step
Protein A/G
Specification
Miapaca2
Read More

Abcam user community

Verified customer

投稿 Jun 16 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab5 antibody - Early Endosome Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Human Cell (ovarian carcinoma (A2780 cell line))
Specification
ovarian carcinoma (A2780 cell line)
Permeabilization
Yes - 0.1% triton X for 5 min at RT
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Aug 26 2014

Western blot abreview for Anti-Rab5 antibody - Early Endosome Marker

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Treatment
20 ug/ml poly(I:C)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Jul 03 2014

Immunocytochemistry/ Immunofluorescence

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Human Cell (Hepatoma)
Specification
Hepatoma
Permeabilization
Yes - 0.2% Triton X-100
Fixative
Formaldehyde
Read More

Ms. Alina Deliu

Verified customer

投稿 May 21 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab5 antibody - Early Endosome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat astrocytes)
Specification
Rat astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Read More

DR. Ruma Raha-Chowdhury

Verified customer

投稿 Dec 04 2012

Question

Hi Abcam,

The customer has advised;

I would like a credit note for each of the antibodies that I have.

Thank you for your help

Kind regards,

Read More

Abcam community

Verified customer

Asked on Aug 30 2012

Answer

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit note ID: ######

As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

Read More

Abcam Scientific Support

Answered on Aug 30 2012

Question

Please see below for the response from the customer;

I finally did the staining with the optimised conditions suggested by Abcam, however I see absolutely no staining at all.

I added 0.2% Tween 20 to my wash and dilution buffers and kept the primary antibody overnight and 4 degrees (1:2000 dilution). I haven't attached any pictures as the background is so faint, that I would have to do a 10 sec exposure to see anything.

If however, Abcam wants a picture, I would be happy to send them some.

Hope this problem can be resolved soon!

Thanks for your help

Read More

Abcam community

Verified customer

Asked on Aug 29 2012

Answer

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange afree of charge replacementor credit note in compensation for each antibody.

I look forward to hearing from you with details of how you would like to proceed.

Read More

Abcam Scientific Support

Answered on Aug 29 2012

1-10 of 35 Abreviews or Q&A

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