Anti-Rab5A 抗体 [3A4] - Early Endosome Marker (ab66746)
Key features and details
- Mouse monoclonal [3A4] to Rab5A - Early Endosome Marker
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Rab5A antibody [3A4] - Early Endosome Marker
Rab5A 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3A4] to Rab5A - Early Endosome Marker -
由来種
Mouse -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Rab5a aa 1-250.
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ポジティブ・コントロール
- WB: Extracts of mouse and human brain. ICC/IF: U87MG cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
3A4 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab66746の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
ターゲット情報
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機能
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
細胞内局在
Cell membrane. Early endosome membrane. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane. Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). - Information by UniProt
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参照データベース
- Entrez Gene: 5868 Human
- SwissProt: P20339 Human
- Unigene: 475663 Human
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別名
- RAB5A antibody
- RAB5A_HUMAN antibody
- Ras-related protein Rab-5A antibody
画像
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hu brain whole cell lysate (20 µg)
Lane 4: Ms brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.This image was produced using the ascites version of this antibody.
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Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + mouse brain at 45 µg
Secondary
goat anti-mouse secondary antibody conjugated to HRP
Predicted band size: 24 kDa -
Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + extracts of mouse brain at 45 µg
Secondary
HRP-conjugated goat anti-mouse
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 24 kDaThis image was produced using the ascites version of this antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab66746 は 9 報の論文で使用されています。
- Patel M et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. Br J Cancer 126:1704-1714 (2022). PubMed: 35173303
- Tang Y et al. The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles. Acta Pharm Sin B 12:2043-2056 (2022). PubMed: 35847504
- Gorji-Bahri G et al. RAB5A effect on metastasis of hepatocellular carcinoma cell line via altering the pro-invasive content of exosomes. Exp Mol Pathol 120:104632 (2021). PubMed: 33831402
- Gorji-Bahri G et al. RAB5A is associated with genes involved in exosome secretion: Integration of bioinformatics analysis and experimental validation. J Cell Biochem 122:425-441 (2021). PubMed: 33225526
- Polla DL et al. Biallelic variants in TMEM222 cause a new autosomal recessive neurodevelopmental disorder. Genet Med 23:1246-1254 (2021). PubMed: 33824500
- Nanou A et al. Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis. Cell Rep 35:109168 (2021). PubMed: 34038728
- Huang HW & Ryoo HD Drosophila fabp is required for light-dependent Rhodopsin-1 clearance and photoreceptor survival. PLoS Genet 17:e1009551 (2021). PubMed: 34714826
- Winer H et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Nat Commun 9:3744 (2018). PubMed: 30218067
- Del Cid-Pellitero E et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. Front Syst Neurosci 11:17 (2017). PubMed: 28408870