Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (Bovine Brain Microvascular Endothelial Cells)
Permeabilization
Yes - Saponin 0.1%
Specification
Bovine Brain Microvascular Endothelial Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 1% in saponin 0.1%
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Alexa Fluor® 568 goat anti-rabbit IgG (H+L) 2 mg/m
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
We have obtained similar results with triton 0.1% as permeabilizing agent.
Abcam response
Thank you for your honest feedback. Cow is an untested species so far and it looks like this antibody may not be suitable for this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Cyril Mougin
Verified customer
投稿 Sep 07 2010