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Anti-Rab4 抗体 - Early Endosome Marker (ab13252)

Reviews (6)Q&A (5)References (34)
Immunocytochemistry - Anti-Rab4 antibody - Early Endosome Marker (ab13252)
  • Western blot - Anti-Rab4 antibody - Early Endosome Marker (ab13252)

Key features and details

  • Rabbit polyclonal to Rab4 - Early Endosome Marker
  • Suitable for: WB, ICC
  • Reacts with: Rat
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab108974)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Rab4 antibody - Early Endosome Marker
    Rab4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Rab4 - Early Endosome Marker

  • 由来種

    Rabbit

  • 特異性

    This antibody is very specific for Rab4. To our knowledge this antibody does not cross-react with other Rab proteins. However, as there are dozens of different proteins in the Rab family, not every single one has been tested for cross-reactivity with this antibody.

  • アプリケーション

    適用あり: WB, ICCmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Fusion protein corresponding to Human Rab4.
    Database link: P20338

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13252の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa).
ICC (1)
1/200 - 1/500.
特記事項
WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa).
ICC
1/200 - 1/500.

ターゲット情報

  • 機能

    Protein transport. Probably involved in vesicular traffic.

  • 配列類似性

    Belongs to the small GTPase superfamily. Rab family.

  • 翻訳後修飾

    Phosphorylated by CDK1 kinase during mitosis.

  • 細胞内局在

    Membrane. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.
  • Information by UniProt

  • 参照データベース

    • 別名

      • HRES 1 / RAB4 antibody
      • Oncogene RAB4 antibody
      • Rab 4 antibody
      • RAB 4A antibody
      • RAB4 member RAS oncogene family antibody
      • Rab4a antibody
      • RAB4A member RAS oncogene family antibody
      • RAB4A_HUMAN antibody
      • Ras related protein Rab 4A antibody
      • Ras related protein Rab4A antibody
      • Ras-related protein Rab-4A antibody
      see all

    画像

    • Immunocytochemistry - Anti-Rab4 antibody - Early Endosome Marker (ab13252)
      Immunocytochemistry - Anti-Rab4 antibody - Early Endosome Marker (ab13252)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

      ab13252, rabbit polyclonal to Rab4 was used in fixed primary cultures of rat hippocampal neurons (embryonic day 18) at a dilution of 1/200. A secondary goat anti-rabbit antibody was used for detection (Alexa Fluor 488; 1/200; green). Confocal microscopy revealed typically punctuate immunofluorescence expected of Rab4 in these neurons. The red colour labelling is that of monoclonal mouse anti-beta-tubulin II at a 1/400 dilution, followed by secondary goat anti-mouse Alexa Fluor 568.

    • Western blot - Anti-Rab4 antibody - Early Endosome Marker (ab13252)
      Western blot - Anti-Rab4 antibody - Early Endosome Marker (ab13252)
      Anti-Rab4 antibody - Early Endosome Marker (ab13252) at 1/1000 dilution + Lysates prepared from rat brain tissue

      Predicted band size: 25.6 kDa

    参考文献 (34)

    ab13252 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab13252 は 34 報の論文で使用されています。

    • Souza ILM  et al. Endocytosis of the non-catalytic ADAM23: Recycling and long half-life properties. Exp Cell Res 398:112415 (2021). PubMed: 33296662
    • Khera L  et al. The AXL-PYK2-PKCa axis as a nexus of stemness circuits in TNBC. Life Sci Alliance 4:N/A (2021). PubMed: 33785524
    • Pazzaglia S  et al. Out-of-Field Hippocampus from Partial-Body Irradiated Mice Displays Changes in Multi-Omics Profile and Defects in Neurogenesis. Int J Mol Sci 22:N/A (2021). PubMed: 33924260
    • Kawamura N  et al. Rab7-Mediated Endocytosis Establishes Patterning of Wnt Activity through Inactivation of Dkk Antagonism. Cell Rep 31:107733 (2020). PubMed: 32521258
    • Noch EK  et al. Distribution and localization of phosphatidylinositol 5-phosphate, 4-kinase alpha and beta in the brain. J Comp Neurol N/A:N/A (2020). PubMed: 32449185
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 11 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab4 antibody - Early Endosome Marker

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HRMEC)
    Permeabilization
    Yes - 0.1% Triton in PBS
    Specification
    HRMEC
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 23 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab4 antibody - Early Endosome Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (COS7)
    Permeabilization
    Yes - 0.2%Triton
    Specification
    COS7
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 07 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab4 antibody - Early Endosome Marker

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (bEnd.3)
    Permeabilization
    Yes - 0.1% Triton in PBS%
    Specification
    bEnd.3
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 03 2015

    Western blot abreview for Anti-Rab4 antibody - Early Endosome Marker

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% SDS-PAGE gel)
    Loading amount
    25 µg
    Specification
    HEK293T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 28 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab4 antibody - Early Endosome Marker

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (Bovine Brain Microvascular Endothelial Cells)
    Permeabilization
    Yes - Saponin 0.1%
    Specification
    Bovine Brain Microvascular Endothelial Cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    MR. Cyril Mougin

    Verified customer

    投稿 Sep 07 2010

    Immunocytochemistry abreview for Anti-Rab4 antibody - Early Endosome Marker

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rat Cell (Hippocampal neuron culture)
    Specification
    Hippocampal neuron culture
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 5%
    Fixative
    Paraformaldehyde
    Read More

    Dr. Randal Moldrich

    Verified customer

    投稿 Dec 07 2005

    Question

    Was a full length recombinant protein used as the immunogen for this antibody, or was a fragment of the protein used?

    Read More
    Answer

    The antibody was raised against the whole molecule of human Rab4 and was expressed in E.coli.

    Read More

    Question

    Testing mouse samples in ICC.    

    Read More
    Answer

    Thank you for the further information. It is difficult to select alternatives that are the most 'specific' as we have not tested these antibodies side by side in the same sample application or species. Reviewing the datasheets I think that ab74906 anti-Rab7 antibody and ab3612 anti-Rabb11 antibody may be preferable but please do check the datasheets to confirm whether you agree. Please bear in mind that ab74906 (rabbit polyclonal to Rab7) is not tested in ICC, and as it shows a weak band in WB the staining intensity may be lower than other antibodies. In addition, ab3612 (rabbit polyclonal to Rab11) is only predicted to work in mouse samples, and may not give positive results. As the Rab7 and Rab11 antibodies will be used in previously untested species/applications, would it be possible to submit an Abreview with your results? It doesn't matter if it is positive or negative, but the information will be really useful for other researchers that may be interested in using these products in a similar manner. I will be able to provide ab78970 and ab87802. Could you also submit Abreview information for these products too? Any other information you be able to share via our Abreview system would be extremely useful. Looking forward to your reply.

    Read More

    Question

    I am concerned that the Rab4 antibody might also recognize other Rab proteins in the cell. Therefore I have a question whether Rab4 antibody offered by your company is specific enough.

    Read More
    Answer

    To our knowledge, this antibody does not cross-react with other Rab proteins. However, as there are dozens of different proteins in the Rab family, not every single one has been tested for cross-reactivity with this antibody. To the best of our knowledge this antibody is very specific for Rab4. Please contact us again if you have any additional questions.

    Read More

    Question

    Hello, if i want to make an immunprecipitation with this antibodie - please can yozu tell me if it recognize the whole endosome? Or only a part of it? Thank you for your help!

    Read More
    Answer

    The antibody ab13252 is specific for Rab4, a 25 kDa GTP-binding protein that regulates recycling of proteins from the early endosomes to the cell surface and therefore will not recognise the whole endosome, I hope this information helps,

    Read More

    1-10 of 11 Abreviews or Q&A

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