Anti-Rab3D 抗体 [EPR8106] (ab128997)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8106] to Rab3D
- Suitable for: mIHC, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Rab3D antibody [EPR8106]
Rab3D 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8106] to Rab3D -
由来種
Rabbit -
アプリケーション
適用あり: mIHC, WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Rab3D aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- BxPC-3, T.T, A549, or HT-29 lysate, Human clear cell carcinoma tissue, Human stomach tissue. mIHC: Human stomach tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 3.36 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8106 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128997の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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mIHC |
Use at an assay dependent concentration.
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WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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mIHC
Use at an assay dependent concentration. |
WB
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Protein transport. Probably involved in regulated exocytosis. -
組織特異性
Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated. -
配列類似性
Belongs to the small GTPase superfamily. Rab family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9545 Human
- Omim: 604350 Human
- SwissProt: O95716 Human
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別名
- D2-2 antibody
- Glioblastoma overexpressed antibody
- GOV antibody
see all
画像
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Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Chromogranin A (ab254557, gray; Opal™690), anti-MUC-6 (ab223846, green; Opal™520) and anti-Rab3D (ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Chromogranin A stained on neuroendocrine cells. Panel D: anti-Rab3D stained on Chief cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab254557 (1/5000), ab223846 (1/1000), and ab128997 (1/10000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
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Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Mucin 5AC (ab3649, gray; Opal™690), anti-MUC-6 (ab223846, green; Opal™520) and anti-Rab3D (ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Mucin 5AC stained on surface mucous cells. Panel D: anti-Rab3D stained on Chief cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab3649 (1/5000 dilution), ab223846 (1/1000 dilution), and ab128997 (1/10000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
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ab128997, at a 1/250 dilution, staining Human Rab3D in clear cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Rab3D antibody [EPR8106] (ab128997) at 1/10000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : T.T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
ab128997, at a 1/250 dilution, staining Human Rab3D in stomach, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab128997 は 3 報の論文で使用されています。
- Jin T et al. Lcn2-derived Circular RNA (hsa_circ_0088732) Inhibits Cell Apoptosis and Promotes EMT in Glioma via the miR-661/RAB3D Axis. Front Oncol 10:170 (2020). PubMed: 32154171
- Ren Y et al. MicroRNA-761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D. Exp Ther Med 17:1841-1846 (2019). PubMed: 30783458
- Cao K et al. The lncRNA HOXA11-AS regulates Rab3D expression by sponging miR-125a-5p promoting metastasis of osteosarcoma. Cancer Manag Res 11:4505-4518 (2019). PubMed: 31191012