Application
Western blot
Sample
Pacific oyster Cell lysate - whole cell (Gills proteome)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
25 µg
Specification
Gills proteome
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: GAR-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
not used
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: ~22 kDa
Positive control
not used
Additional data
Additional Notes
We tested for differential regulation of Rab11 in oysters proteome for two different cases-wild (W) and treated (Q). A relative higher intensity was observed in Q as can be seen visually from the image. Quantitation also showed an increased intensity. Given the fact that homology of the antibodies with oysters proteins was not great (88%) the result was very promising.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 28 2017