Anti-RAB11B 抗体 (ab3612)

Reviews (8)Q&A (8)References (25)
Immunocytochemistry - Anti-RAB11B antibody (ab3612)
  • Western blot - Anti-RAB11B antibody (ab3612)
  • Western blot - Anti-RAB11B antibody (ab3612)

Key features and details

  • Rabbit polyclonal to RAB11B
  • Suitable for: ICC, WB
  • Reacts with: Dog, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-RAB11B antibody [EPR12719] (ab176344)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-RAB11B antibody
    RAB11B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to RAB11B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, WBmore details

  • 種交差性

    交差種: Dog, Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Non human primates

  • 免疫原

    Synthetic peptide corresponding to Human RAB11B aa 1-100.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: canine MDCK, human PC3; ICC: HeLa

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3612の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
1/50 - 1/200.
WB (2)
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.
特記事項
ICC
1/50 - 1/200.
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa). By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.

ターゲット情報

  • 機能

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis.

  • 配列類似性

    Belongs to the small GTPase superfamily. Rab family.

  • 翻訳後修飾

    Citrullinated by PADI4.

  • 細胞内局在

    Recycling endosome membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, phagosome membrane. Recruited to phagosomes containing S.aureus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • GTP-binding protein ypt3 antibody
    • H YPT3 antibody
    • RAB11B antibody
    • RAB11B member RAS oncogene family antibody
    • Ras-related protein Rab-11B antibody
    • RB11B_HUMAN antibody
    • YPT3 antibody
    see all

画像

  • Immunocytochemistry - Anti-RAB11B antibody (ab3612)
    Immunocytochemistry - Anti-RAB11B antibody (ab3612)

    Immunocytochemical analysis of 4% paraformaldeehyde-fixed 0.1% Triton X-100 permeabilized HeLa cells treated/untreated with actinomycin D. Rab11B is stained with ab3612 at a 1/100 dilution and a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate at a 1/500 dilution (green). Nuclear counterstaining is using Hoechst 33342 (blue).

  • Western blot - Anti-RAB11B antibody (ab3612)
    Western blot - Anti-RAB11B antibody (ab3612)
    Anti-RAB11B antibody (ab3612) at 1/500 dilution + MDCK (canine kidney cell line) whole cell lysate at 20 µg

    Predicted band size: 24 kDa

  • Western blot - Anti-RAB11B antibody (ab3612)
    Western blot - Anti-RAB11B antibody (ab3612)

    ab3612 used on PC3 cell extract.

参考文献 (25)

ab3612 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3612 は 25 報の論文で使用されています。

  • Hou J  et al. Glycoprotein 5 Is Cleaved by Cathepsin E during Porcine Reproductive and Respiratory Syndrome Virus Membrane Fusion. J Virol 94:N/A (2020). PubMed: 32102888
  • Parks XX  et al. Fluvastatin inhibits Rab5-mediated IKs internalization caused by chronic Ca2+-dependent PKC activation. J Mol Cell Cardiol 129:314-325 (2019). PubMed: 30898664
  • Markworth R  et al. Sensory Axon Growth Requires Spatiotemporal Integration of CaSR and TrkB Signaling. J Neurosci 39:5842-5860 (2019). PubMed: 31123102
  • Vantaggiato C  et al. ZFYVE26/SPASTIZIN and SPG11/SPATACSIN mutations in hereditary spastic paraplegia types AR-SPG15 and AR-SPG11 have different effects on autophagy and endocytosis. Autophagy 15:34-57 (2019). PubMed: 30081747
  • Ma W  et al. The Vacuolar Pathway of Long Peptide Cross-Presentation Can Be TAP Dependent. J Immunol 202:451-459 (2019). PubMed: 30559321
View all Publications for this product

レビューと Q&A

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1-10 of 16 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-RAB11B antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hela)
Specification
hela
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More

Dr. Amani Hassan

Verified customer

投稿 May 09 2017

Western blot abreview for Anti-RAB11B antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Pacific oyster Cell lysate - whole cell (Gills proteome)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
25 µg
Specification
Gills proteome
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Apr 28 2017

Western blot abreview for Anti-RAB11B antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Hamster Cell lysate - whole cell (Baby hamster kidney fibroblast cells (BHK-21))
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
10 µg
Specification
Baby hamster kidney fibroblast cells (BHK-21)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Mar 02 2016

IHC - Wholemount abreview for Anti-RAB11B antibody

 Inconclusive
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Drosophila melanogaster Tissue (Follicular epithelium)
Specification
Follicular epithelium
Read More

Mr. Veit Riechmann

Verified customer

投稿 May 14 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-RAB11B antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Kidney (ATCC CCL-34))
Permeabilization
Yes - 0.5% saponin
Specification
Kidney (ATCC CCL-34)
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Oct 07 2011

Question


Inquiry: I am curious as to whether your Anti-Rab11 antibody is specific to Rab11A, Rab11B, or both?

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Answer

Thank you for contacting us.

This antibody detects both RAB11A and Rab11B.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question


The cells I am using are HEK293 cells, so they are human. The protein concentration is unknown (I haven’t quantitated it), however, the same sample was used for Rab5 (ab18211, a marker for early endosomes) and beta-actin (ab8227), and those antibodies worked well. I am assuming there would be realitvely similar quantities of late (Rab7) and recycling (Rab11) endosomes to early endosomes (Rab5), but perhaps I am mistaken?

The lysis buffer I am currently using is M-PER Mammalian Protein Extract Reagent from Thermo Scientific, so naturally the recipe is proprietary.

I have been leaving the primary antibody on for 1hr, at room temperature, on a rocking platform

I was originally blocking in 3% BSA overnight, but switched to 1% BSA for 1hr and still am seeing no bands

Yes, the secondary antibody I am using has worked with Rab5 and beta-actin
Any suggestions would be very useful!

Read More
Answer

Thank you for your reply. I would recommend keeping the blocking to one hour and incubating the primary antibody overnight at 4 degrees. I would suggest doing this at 1/300 for ab74906 and at 1/1000 for ab3612. Please let me know if this does not improve your results.

Read More

Question

Inquiry: Dear Sir or Madam, I am currently looking for an anti-human Rab 11 antibody for immunocytochemistry to perform localization studies. Unfortunately, we have had negative experience with antibodies that were not specific or not sensitive enough for confocal imaging of Rab11. Are there any means to test the antibody before buying the lot? We would be very grateful if it was possible to obtain a free tester sample sufficient for staining 2 - 3 coverslips of the anti-Rab 11 rabbit polyclonal antibody (ab3612). As our lab is buying quite frequently at abcam, we could arrange including the sample into next package to avoid extra shipping payments. I will greatly appreciate receiving any information on anti-Rab 11 antibodies (advise, new products, etc.) that you can provide. Yours sincerely,

Read More
Answer

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

As shown on the product datasheet, the Rabbit polyclonal anti-Rab11 ab3612 (https://www.abcam.com/ab3612) is guaranteed to work in ICC/IF, Western Blot and on Human, Dog, Non Human Primates samples.
The other anti-Rab11 we have in our catalogue is a Rat monoclonal, reference ab95375 (https://www.abcam.com/ab95375). Like ab3612 it has been tested in ICC/IF and Western Blot but only on Mouse samples.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Question

Testing mouse samples in ICC.    

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Answer

Thank you for the further information. It is difficult to select alternatives that are the most 'specific' as we have not tested these antibodies side by side in the same sample application or species. Reviewing the datasheets I think that ab74906 anti-Rab7 antibody and ab3612 anti-Rabb11 antibody may be preferable but please do check the datasheets to confirm whether you agree. Please bear in mind that ab74906 (rabbit polyclonal to Rab7) is not tested in ICC, and as it shows a weak band in WB the staining intensity may be lower than other antibodies. In addition, ab3612 (rabbit polyclonal to Rab11) is only predicted to work in mouse samples, and may not give positive results. As the Rab7 and Rab11 antibodies will be used in previously untested species/applications, would it be possible to submit an Abreview with your results? It doesn't matter if it is positive or negative, but the information will be really useful for other researchers that may be interested in using these products in a similar manner. I will be able to provide ab78970 and ab87802. Could you also submit Abreview information for these products too? Any other information you be able to share via our Abreview system would be extremely useful. Looking forward to your reply.

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Question

I am going to titrate the monoclonal Rab11 antibody (ab3612) with my cell for IF. Would you suggest to series dilute the secondary antibody or keep its amount constant for different concentrations of primary antibody, when the secondary antibody is assumed quite specific?

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Answer

Thank you for contacting us. Yes, a suggested starting dilution for the primary antibody would be 1/200. And I would suggest keeping the secondary antibody dilution constant while titrating the primary antibody. Once you know the optimal dilution for the primary you could try a higher and a lower dilution of the secondary to see if any other dilution might give even better results. But it is often advisable to change only one parameter the time. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 16 Abreviews or Q&A

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