製品の概要

  • 製品名
    Anti-RAB10 (phospho T73) antibody [MJF-R21]
    RAB10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [MJF-R21] to RAB10 (phospho T73)
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, Dot blotmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide within Human RAB10 aa 50-150 (phospho T73). The exact sequence is proprietary.
    Database link: P61026

  • ポジティブ・コントロール
    • WB: Wild-type MEF whole cell lysate; LRRK2 [R1441C] knock-in MEF whole cell lysate; Wild-type whole cell lysate untreated and treated with 100 nM MLi-2 for 90 min; Rab8A knock-out whole cell lysate untreated and treated with 100 nM MLi-2 for 90 min; HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab10 expression vectors, treated with 150 nM MLi-2 for 90 min whole cell lysate.
  • 特記事項

    Please see PMID: 29127256. Lis P  et al. Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase. Biochem J 475:1-22 (2018).

    This antibody was developed with support from The Michael J. Fox Foundation.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab230261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Dot blot 1/1000.

ターゲット情報

  • 機能
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.
  • 配列類似性
    Belongs to the small GTPase superfamily. Rab family.
  • 細胞内局在
    Cytoplasmic vesicle membrane. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cell projection, cilium. Endoplasmic reticulum membrane. Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280).
  • Information by UniProt
  • 参照データベース
  • 別名
    • GTP binding protein RAB10 antibody
    • Rab10 antibody
    • RAB10 member RAS oncogene family antibody
    • RAB10_HUMAN antibody
    • Ras related GTP binding protein antibody
    • Ras related GTP binding protein RAB10 antibody
    • Ras-related protein Rab-10 antibody
    see all

画像

  • All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cells transfected with LRRK2 [Y1699C] and HA-tagged Rab3A expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate
    Lane 2 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab8A expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate
    Lane 3 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab10 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate
    Lane 4 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab35 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate
    Lane 5 : HEK-293 cells transfected with LRRK2[Y1699C] and HA-tagged Rab43 expression vectors, were treated with 150 nM MLi-2 for 90 minutes, whole cell lysate

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : IRDye 800CW secondary antibody at 1/25000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% BSA/TBST.

    The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates the phosphorylation of Rab proteins.

    The images were kindly provided by our collaborator Dr. Dario Alessi, and have been published (PMID: 29127256).

    Scanned with Licor Odyssey CLx.

  • All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

    Lane 1 : Wild-type A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : Wild-type A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate
    Lane 3 : Rab8A knock-out A549 whole cell lysate
    Lane 4 : Rab8A knock-out A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate
    Lane 5 : Rab10 knock-out A549 whole cell lysate
    Lane 6 : Rab10 knock-out A549 treated with 100 nM MLi-2 for 90 minutes, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-labeled secondary antibody at 1/2500 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% BSA/TBST.

    The images were kindly provided by our collaborator, Dr. Dario Alessi, and have been published (PMID: 29127256).

    Scanned with Licor Odyssey CLx.

  • All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21] (ab230261) at 1/1000 dilution

    Lane 1 : Wild-type MEF (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : Wild-type MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate
    Lane 3 : LRRK2 [R1441C] knock-in MEF whole cell lysate
    Lane 4 : LRRK2 [R1441C] knock-in MEF treated with 100 nM MLi-2 for 90 minutes, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The LRRK2 pathogenic mutation R1441C increases LRRK2 activity and markedly elevates Rab10 phosphorylation in MEF (mouse embryonic fibroblasts).

    The expression pattern is consistent with the literature (PMID: 29127256).

    The cell lysates were kindly provided by our collaborator, Dr. Dario Alessi.

  • Dot blot analysis of Rab10 (phospho T73) labeled with ab230261 at 1/1000 dilution.

    Lane 1: Rab10 (phospho T73) peptide;

    Lane 2: Rab10 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 32 seconds.

参考文献

This product has been referenced in:
  • Lis P  et al. Development of phospho-specific Rab protein antibodies to monitorin vivoactivity of the LRRK2 Parkinson's disease kinase. Biochem J 475:1-22 (2018). Read more (PubMed: 29127256) »
  • Fan Y  et al. Interrogating Parkinson's disease LRRK2 kinase pathway activity by assessing Rab10 phosphorylation in human neutrophils. Biochem J 475:23-44 (2018). Read more (PubMed: 29127255) »
See all 5 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab230261.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

登録