製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RAB10
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 96-125 of Human RAB10 (NP_057215.3).

  • ポジティブ・コントロール
    • A2058 cell line lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 22 kDa.

ターゲット情報

  • 機能
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.
  • 配列類似性
    Belongs to the small GTPase superfamily. Rab family.
  • 細胞内局在
    Cytoplasmic vesicle membrane. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cell projection, cilium. Endoplasmic reticulum membrane. Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280).
  • Information by UniProt
  • 参照データベース
  • 別名
    • GTP binding protein RAB10 antibody
    • Rab10 antibody
    • RAB10 member RAS oncogene family antibody
    • RAB10_HUMAN antibody
    • Ras related GTP binding protein antibody
    • Ras related GTP binding protein RAB10 antibody
    • Ras-related protein Rab-10 antibody
    see all

画像

  • Anti-RAB10 antibody (ab110517) at 1/100 dilution + A2058 cell line lysates at 35 µg

    Predicted band size: 22 kDa

プロトコール

参考文献

ab110517 has not yet been referenced specifically in any publications.

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Answer

I am very pleased to hear you would like to accept our offer and test ab110517 in drosophila. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for drosophila and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for your inquiry. Based on the BLAST results, the immunogen sequence used to generate ab104859 is only 69% homologous with drosophila. This is a bit low, so cross-reactivity with drosophila would probably not be expected with this antibody. Our other RAB10 antibodies, ab110517 and ab113947, have 90% and ~60% homologies, respectively, with drosophila. Therefore, I would recommend ab110517 since this has the highest homology, but again since it hasn't been tested in drosophila we wouldn't be able to guarantee cross-reactivity. To our knowledge, ab110517 has not been tested in drosophila. Therefore, I can offer a discount off a future purchase if you buy ab110517 now, test it in drosophila and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab110517 in drosphila. I will then send a discount code. This code must be issued before purchasing ab110517 so please wait for my reply before ordering. 2. Purchase ab110517 either by phone, fax, or online (www.abcam.com). 3. Test it in drosophila. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab110517 turns out to be unsuitable for drosophila, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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