Application
Western blot
Sample
Rat Cell lysate - other (Rat hepatocytes plasma membrane fraction)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
35 µg
Specification
Rat hepatocytes plasma membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey-anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
45 second(s)
Bands
Specific: around 28 kDa kDa Non-specific: 75 kDa kDa
Additional data
Additional Notes
MW of rat Rab6a is 26 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
投稿 Oct 29 2014