Anti-PYK2 (phospho Y579 + Y580) 抗体 (ab4807)

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Western blot - Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)

    Key features and details

    • Rabbit polyclonal to PYK2 (phospho Y579 + Y580)
    • Suitable for: WB
    • Reacts with: Rat, Chicken, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-PYK2 (phospho Y579 + Y580) antibody
      PYK2 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to PYK2 (phospho Y579 + Y580)

    • 由来種

      Rabbit

    • 特異性

      Some cross-reactivity still may be experienced in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 within the same cell system.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Rat, Chicken, Human

    • 免疫原

      Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosines 579 and 580. The sequence is conserved in human and rat.

    • ポジティブ･コントロール

      • Vandate treated CEF cells expressing PYK 2 and plated on fibronectin.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.3
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2, (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence, and (iii) a phosphopeptide derived from the corresponding region of Focal Adhesion Kinase (a PYK 2-related protein) to remove antibody that is reactive with phosphorylated Focal Adhesion Kinase protein. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosines 579 and 580.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab4807 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB
  • 追加情報
    WB: 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with PYK2 peptide - phospho Y579 + Y580 (phospho and non-phospho pair) or CEF whole cell lysate, positive / negative for PYK 2 (pair).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能

      Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.

    • 組織特異性

      Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.

    • 配列類似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.

    • 翻訳後修飾

      Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.

    • 細胞内局在

      Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CADTK antibody
        • CAK-beta antibody
        • CAKB antibody
        • CAKbeta antibody
        • Calcium regulated non receptor proline rich tyrosine kinase antibody
        • Calcium-dependent tyrosine kinase antibody
        • Cell adhesion kinase beta antibody
        • E430023O05Rik antibody
        • EC 2.7.10.2 antibody
        • FADK 2 antibody
        • FADK2 antibody
        • FAK2 antibody
        • FAK2_HUMAN antibody
        • Focal adhesion kinase 2 antibody
        • MGC124628 antibody
        • PKB antibody
        • Proline-rich tyrosine kinase 2 antibody
        • Protein kinase B antibody
        • Protein Tyrosine Kinase 2 Beta antibody
        • Protein-tyrosine kinase 2-beta antibody
        • PTK antibody
        • PTK2B antibody
        • PTK2B protein tyrosine kinase 2 beta antibody
        • PYK2 antibody
        • RAFTK antibody
        • RAFTK2 antibody
        • Related adhesion focal tyrosine kinase antibody
        see all

      画像

      • Western blot - Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)
        Western blot - Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)
        Peptide Competition: Cell extracts prepared from chick embryo fibroblasts treated with vanadate were plated on fibronectin with (lanes 1-5) or without (lane 6) transfection of a PYK 2 expression vector and resolved by SDS PAGE on a 10% Tris-glycine gel. The proteins were then transferred to nitrocellulose. Membranes were incubated with 0.50 µg/mL ab4807, following prior incubation in the presence of the phosphopeptide immunogen (1), the absence of the phosphopeptide immunogen (2, 6), the non phosphopeptide corresponding to the PYK 2 phosphopeptide (3), the phosphopeptide corresponding to PYK 2 [pY579] (4), and the phosphopeptide corresponding to PYK 2 [pY580] (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the dual-phosphopeptide corresponding to this site blocks the antibody signal, not the corresponding mono phosphopeptides, t

      参考文献 (0)

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      ab4807 は論文での使用が確認できていません。

      レビューと Q&A

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      レビューを送る 質問を送る

      Question

      Order confirmation, FAK cross reactivity data

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      Answer

      On gel systems that resolve FAK (pp125) and Pyk2 (pp116), they will show up as a distinct doublet. If there is a lot of FAK relative to Pyk2, the normally low level of cross-reactivity (due to the fact that the antibodies are negatively adsorbed with the irrelevant phosphopeptides during our manufacturing process) can become a problem. For example, if FAK is present at levels 20-fold greater than Pyk2, then even if the Pyk2 antibodies are 95% selective for Pyk2 vs. FAK, you would see an equal signal for the two proteins.
      The reactivity for FAK is removed by absorption with a phosphopeptide with a corresponding sequence in FAK. This kind of adsorption will not be 100% that is why in the cells overexpress with FAK you will see signal.

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