Anti-PYK2 (phospho Y579 + Y580) 抗体 (ab4807)
Key features and details
- Rabbit polyclonal to PYK2 (phospho Y579 + Y580)
- Suitable for: IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PYK2 (phospho Y579 + Y580) antibody
PYK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PYK2 (phospho Y579 + Y580) -
由来種
Rabbit -
特異性
Some cross-reactivity still may be experienced in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 within the same cell system. -
アプリケーション
適用あり: IHC-P, ICCmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosines 579 and 580. The sequence is conserved in human and rat.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.3
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2, (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence, and (iii) a phosphopeptide derived from the corresponding region of Focal Adhesion Kinase (a PYK 2-related protein) to remove antibody that is reactive with phosphorylated Focal Adhesion Kinase protein. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosines 579 and 580. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4807の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/10 - 1/100.
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| ICC |
1/250.
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| 特記事項 |
|---|
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IHC-P
1/10 - 1/100. |
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ICC
1/250. |
ターゲット情報
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機能
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption. -
組織特異性
Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. -
細胞内局在
Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase. - Information by UniProt
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参照データベース
- Entrez Gene: 2185 Human
- Entrez Gene: 19229 Mouse
- Omim: 601212 Human
- SwissProt: Q14289 Human
- SwissProt: Q9QVP9 Mouse
- Unigene: 491322 Human
- Unigene: 21613 Mouse
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別名
- CADTK antibody
- CAK-beta antibody
- CAKB antibody
see all
画像
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Immunocytochemistry - Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)
Immunocytochemistry analysis of A549 cells labelling PYK2 with ab4807 at 1/250 dilution. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. A Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate was used as a secondary antibody at 1:2000. Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing membranous localization. Panel e is untreated cell with no signal. Panel f is a no primary antibody control. The images were captured at 60X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)Immunohistochemistry analysis of human spleen tissue labelling Phospho-Pyk2 (pYpY579/580) with ab4807 at 1/20 dilution. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. A no was also produced antibody control (left).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)Immunohistochemistry analysis of mouse brain tissue labelling Phospho-Pyk2 (pYpY579/580) with ab4807 at 1/20 dilution. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. A no was also produced antibody control (left).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
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