Anti-PYGL 抗体 (ab198268)
Key features and details
- Rabbit polyclonal to PYGL
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PYGL antibody
PYGL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PYGL -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment (GST-tag) corresponding to Human PYGL (C terminal). BC082229
Database link: P06737 -
ポジティブ・コントロール
- 231 and PC3 cell lysates. Human thyroid cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab198268の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
1/30 - 1/150.
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WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 97 kDa.
|
特記事項 |
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IHC-P
1/30 - 1/150. |
WB
1/500 - 1/2000. Predicted molecular weight: 97 kDa. |
ターゲット情報
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機能
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. -
関連疾患
Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6) [MIM:232700]. A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected. -
配列類似性
Belongs to the glycogen phosphorylase family. -
翻訳後修飾
Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A. - Information by UniProt
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参照データベース
- Entrez Gene: 5836 Human
- Entrez Gene: 110095 Mouse
- Entrez Gene: 64035 Rat
- Omim: 613741 Human
- SwissProt: P06737 Human
- SwissProt: Q9ET01 Mouse
- SwissProt: P09811 Rat
- Unigene: 282417 Human
see all -
別名
- Glycogen phosphorylase antibody
- Glycogen phosphorylase L antibody
- Glycogen phosphorylase liver antibody
see all
画像
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All lanes : Anti-PYGL antibody (ab198268) at 1/400 dilution
Lane 1 : 231 cell lysate
Lane 2 : PC3 cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 20 seconds -
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PYGL with ab198268 at 1/35 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab198268 は 9 報の論文で使用されています。
- Sadiku P et al. Neutrophils Fuel Effective Immune Responses through Gluconeogenesis and Glycogenesis. Cell Metab 33:411-423.e4 (2021). PubMed: 33306983
- Xu JH et al. Up-Regulation of Glycogen Synthesis and Degradation Enzyme Level Maintained Myocardial Glycogen in Huddling Brandt's Voles Under Cool Environments. Front Physiol 12:593129 (2021). PubMed: 33841168
- Xu J et al. Long noncoding RNA KCNMB2-AS1 promotes the development of esophageal cancer by modulating the miR-3194-3p/PYGL axis. Bioengineered 12:6687-6702 (2021). PubMed: 34516362
- Kawakubo-Yasukochi T et al. Hepatic glycogenolysis is determined by maternal high-calorie diet via methylation of Pygl and it is modified by oteocalcin administration in mice. Mol Metab 54:101360 (2021). PubMed: 34673295
- Liu C et al. Glycogen Phosphorylase: A Drug Target of Amino Alcohols in Echinococcus granulosus, Predicted by a Computer-Aided Method. Front Microbiol 11:557039 (2020). PubMed: 33329421
- Mantini G et al. Co-expression analysis of pancreatic cancer proteome reveals biology and prognostic biomarkers. Cell Oncol (Dordr) 43:1147-1159 (2020). PubMed: 32860207
- Hughey CC et al. Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation. Mol Metab 23:1-13 (2019). PubMed: 30850319
- Hughey CC et al. Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates. J Biol Chem 293:11944-11954 (2018). PubMed: 29891549
- Liang X et al. Organic cation transporter 1 (OCT1) modulates multiple cardiometabolic traits through effects on hepatic thiamine content. PLoS Biol 16:e2002907 (2018). PubMed: 29659562