Anti-Pumilio 1 抗体 (ab3717)

Submit a review Q&A (1)References (3)
Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
  • Western blot - Anti-Pumilio 1 antibody (ab3717)
  • Western blot - Anti-Pumilio 1 antibody (ab3717)

Key features and details

  • Goat polyclonal to Pumilio 1
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Pumilio 1 antibody
    Pumilio 1 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to Pumilio 1

  • 由来種

    Goat

  • アプリケーション

    適用あり: IP, WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant

  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.

  • ポジティブ･コントロール

    • WB: NIH/3T3, HEK293T, A549, K562 whole cell lysate; IP: HEK293T

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3717の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at 2-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).
特記事項
IP
Use at 2-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).

ターゲット情報

画像

  • Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
    Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)

    Pumilio 1 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3717 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3717 at 0.1 µg/mL.

    Lane 1: rabbit anti-Pumillio 1 antibody IP in HEK293T whole cell lysate.

    Lane 2: ab3717 IP in HEK293T whole cell lysate.

    Lane 3: ab3717 IP in HEK293T whole cell lysate

    Lane 4: Control IgG in HEK293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 seconds

  • Western blot - Anti-Pumilio 1 antibody (ab3717)
    Western blot - Anti-Pumilio 1 antibody (ab3717)
    All lanes : Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : A549 whole cell lysate
    Lane 3 : K562 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated rabbit anti-goat IgG

    Predicted band size: 126 kDa


    Exposure time: 10 seconds
  • Western blot - Anti-Pumilio 1 antibody (ab3717)
    Western blot - Anti-Pumilio 1 antibody (ab3717)
    Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml + NIH/3T3 whole cell lysate at 50 µg

    Secondary
    HRP-conjugated rabbit anti-goat IgG

    Predicted band size: 126 kDa


    Exposure time: 10 seconds

参考文献 (3)

ab3717 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3717 は 3 報の論文で使用されています。

  • Sajek M  et al. PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cell Mol Life Sci 76:147-161 (2019). PubMed: 30269240
  • Janecki DM  et al. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget 9:32466-32477 (2018). PubMed: 30197756
  • Marrero E  et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). IHC-Fr ; Mouse . PubMed: 21865157

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

Customer's question: Do you know whether ab3717 cross reacts with Pum2. If you do not know, could you send me the peptide sequence to see if it is likely to cross react. Thank you

Read More
Answer

ab3717 does not cross react with Pum2.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com