Anti-Pumilio 1 抗体 (ab3717)

Submit a review Q&A (1)References (3)
Western blot - Anti-Pumilio 1 antibody (ab3717)

    Key features and details

    • Goat polyclonal to Pumilio 1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Pumilio 1 antibody
      Pumilio 1 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to Pumilio 1

    • 由来種

      Goat

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant

    • 免疫原

      Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab3717 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).

    ターゲット情報

    画像

    • Western blot - Anti-Pumilio 1 antibody (ab3717)
      Western blot - Anti-Pumilio 1 antibody (ab3717)

      Sample: Hela cell total RIPA extract (10µg)

      Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)

      CIP: Calf Intestinal Phosphatase

      Detection: ECL exposure for 1 minute.

      Sample: Hela cell total RIPA extract (10µg)

      Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)

      CIP: Calf Intestinal Phosphatase

      Detection: ECL exposure for 1 minute.

    参考文献 (3)

    ab3717 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3717 は 3 報の論文で使用されています。

    • Sajek M  et al. PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cell Mol Life Sci 76:147-161 (2019). PubMed: 30269240
    • Janecki DM  et al. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget 9:32466-32477 (2018). PubMed: 30197756
    • Marrero E  et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). IHC-Fr ; Mouse . PubMed: 21865157

    レビューと Q&A

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    Question

    Customer's question: Do you know whether ab3717 cross reacts with Pum2. If you do not know, could you send me the peptide sequence to see if it is likely to cross react. Thank you

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    Answer

    ab3717 does not cross react with Pum2.

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