Anti-Pumilio 1 抗体 (ab3717)
Key features and details
- Goat polyclonal to Pumilio 1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Pumilio 1 antibody
Pumilio 1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Pumilio 1 -
由来種
Goat -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant
-
免疫原
Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.
-
ポジティブ･コントロール
- WB: NIH/3T3, HEK293T, A549, K562 whole cell lysate; IP: HEK293T
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3717の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB |
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).
|
| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa). |
ターゲット情報
- Information by UniProt
-
参照データベース
- Entrez Gene: 535450 Cow
- Entrez Gene: 9698 Human
- Entrez Gene: 80912 Mouse
- Entrez Gene: 362609 Rat
- Omim: 607204 Human
- SwissProt: Q14671 Human
- SwissProt: Q80U78 Mouse
- SwissProt: Q5R5X3 Orangutan
see all -
製品の状態
Cytoplasm -
別名
- HsPUM antibody
- KIAA0099 antibody
- PUM 1 antibody
see all
画像
-
Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
Pumilio 1 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3717 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3717 at 0.1 µg/mL.
Lane 1: rabbit anti-Pumillio 1 antibody IP in HEK293T whole cell lysate.
Lane 2: ab3717 IP in HEK293T whole cell lysate.
Lane 3: ab3717 IP in HEK293T whole cell lysate
Lane 4: Control IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds
-
Western blot - Anti-Pumilio 1 antibody (ab3717)All lanes : Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml
Lane 1 : HEK293T whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : K562 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated rabbit anti-goat IgG
Predicted band size: 126 kDa
Exposure time: 10 seconds -
Western blot - Anti-Pumilio 1 antibody (ab3717)Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml + NIH/3T3 whole cell lysate at 50 µg
Secondary
HRP-conjugated rabbit anti-goat IgG
Predicted band size: 126 kDa
Exposure time: 10 seconds
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab3717 は 3 報の論文で使用されています。
- Sajek M et al. PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cell Mol Life Sci 76:147-161 (2019). PubMed: 30269240
- Janecki DM et al. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget 9:32466-32477 (2018). PubMed: 30197756
- Marrero E et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). IHC-Fr ; Mouse . PubMed: 21865157