Application
Western blot
Sample
Rabbit Tissue lysate - whole (brain)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
0.2 µg
Treatment
hypoxia 40 minutes at E25
Specification
brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
30 minute(s) · Temperature: 22°C · Diluent: proteinsimple antibody dilution buffer
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: proteinsimple goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Luminol-S + Peroxide
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 57 kDa Non-specific: 41, 48, 68, 95, 117, 209 kDa
Positive control
NA
Additional data
Additional Notes
AbTrial REFERENCE: 13323226-B
Lane 1: ladder
Lane 2: rabbit brain tissue lysate
Colby - This was used on order 3310043
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 19 2018