Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6715] to PTPRE
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
製品名Anti-PTPRE antibody [EPR6715]
PTPRE 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6715] to PTPRE
アプリケーション適用あり: WB, Flow Cytmore details
適用なし: ICC/IF,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide corresponding to Human PTPRE aa 650-750.
Database link: P23469
- HL60 treated with TPA, HL60, HeLa and C2C12 cell lysates; Permeabilized HL60 cells.
This product was previously labelled as PTP epsilon
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
解離定数（KD 値）KD = 5.10 x 10 -11 M Learn more about KD
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab126788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 71, 75, 81 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
機能Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function.
Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake.
Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).
組織特異性Expressed in giant cell tumor (osteoclastoma rich in multinucleated osteoclastic cells).
配列類似性Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.
Contains 2 tyrosine-protein phosphatase domains.
ドメインThe tyrosine-protein phosphatase 2 domain (D2) mediates dimerization. The extreme N- and C- termini of the D2 domain act to inhibit dimerization and removal of these sequences increases dimerization and inhibits enzyme activity.
翻訳後修飾A catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3.
Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu.
Isoform 1 is glycosylated.
細胞内局在Cytoplasm; Cell membrane and Cytoplasm. Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin induces translocation to the membrane.
- Information by UniProt
- DKFZp313F1310 antibody
- FLJ57799 antibody
- FLJ58245 antibody
All lanes : Anti-PTPRE antibody [EPR6715] (ab126788) at 1/1000 dilution
Lane 1 : HL60 cell lysate, treated with TPA
Lane 2 : HL60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : C2C12 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 71, 75, 81 kDa
ab126788 at 1/10 dilution staining PTPRE in permeabilized HL60 cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).
Equilibrium disassociation constant (KD)
Learn more about KD
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