Anti-PTP1B 抗体 (ab189179)
Key features and details
- Rabbit polyclonal to PTP1B
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PTP1B antibody
PTP1B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PTP1B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Synthetic peptide within Human PTP1B aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_002818.1).
Database link: P18031 -
ポジティブ・コントロール
- Human kidney tissue; Extracts from COS7 cells, treated with UV 30'.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab189179の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 50 kDa.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 50 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
May play an important role in CKII- and p60c-src-induced signal transduction cascades. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
翻訳後修飾
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5770 Human
- Omim: 176885 Human
- SwissProt: P18031 Human
- Unigene: 417549 Human
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別名
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling PTP1B with ab189179 at 10 µg/ml.
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All lanes : Anti-PTP1B antibody (ab189179) at 1/500 dilution
Lane 1 : Extracts from COS7 cells, treated with UV 30'
Lane 2 : Extracts from COS7 cells, treated with UV 30' with immunizing peptide
Predicted band size: 50 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab189179 は 6 報の論文で使用されています。
- Shi H et al. Supplement of microbiota-accessible carbohydrates prevents neuroinflammation and cognitive decline by improving the gut microbiota-brain axis in diet-induced obese mice. J Neuroinflammation 17:77 (2020). PubMed: 32127019
- Shi H et al. ß-glucan attenuates cognitive impairment via the gut-brain axis in diet-induced obese mice. Microbiome 8:143 (2020). PubMed: 33008466
- Shu XS et al. Loss of ß-catenin via activated GSK3ß causes diabetic retinal neurodegeneration by instigating a vicious cycle of oxidative stress-driven mitochondrial impairment. Aging (Albany NY) 12:13437-13462 (2020). PubMed: 32575075
- Guerville M et al. Chronic refined low-fat diet consumption reduces cholecystokinin satiation in rats. Eur J Nutr 58:2497-2510 (2019). PubMed: 30069617
- Garner KL et al. Enhanced insulin receptor, but not PI3K, signalling protects podocytes from ER stress. Sci Rep 8:3902 (2018). PubMed: 29500363
- Borges Bde C et al. Protein tyrosine phosphatase-1B contributes to LPS-induced leptin resistance in male rats. Am J Physiol Endocrinol Metab 308:E40-50 (2015). PubMed: 25352433