Anti-PTGIS/PGIS 抗体 (ab23668)
Key features and details
- Rabbit polyclonal to PTGIS/PGIS
- Suitable for: WB, ICC/IF, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Sheep, Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PTGIS/PGIS antibody
PTGIS/PGIS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PTGIS/PGIS -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Fr, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Sheep, Cow, Human -
免疫原
Synthetic peptide corresponding to Mouse PTGIS/PGIS aa 475-490.
Sequence:PEFDLSRYGFGLMQPE
(Peptide available asab187257) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: None -
Concentration information loading... -
特記事項（精製）
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23668の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration.
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2). -
組織特異性
Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate. -
配列類似性
Belongs to the cytochrome P450 family. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 282021 Cow
- Entrez Gene: 5740 Human
- Entrez Gene: 19223 Mouse
- Entrez Gene: 25527 Rat
- Omim: 601699 Human
- SwissProt: Q29626 Cow
- SwissProt: Q16647 Human
- SwissProt: O35074 Mouse
see all -
別名
- CYP8 antibody
- CYP8A1 antibody
- Cytochrome P450, family 8, subfamily A, polypeptide 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGIS/PGIS antibody (ab23668)
ab23668 (4µg/ml) staining PTGIS/PGIS in human adrenal gland using an automated system (DAKO Autostainer Plus). Using this protocol there is apical cytoplasmic/ endoplasmic reticulum staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
データシートおよび資料
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Datasheet download
参考文献 (10)
ab23668 は 10 報の論文で使用されています。
- Ipseiz N et al. Tissue-resident macrophages actively suppress IL-1beta release via a reactive prostanoid/IL-10 pathway. EMBO J 39:e103454 (2020). PubMed: 32484988
- Ebmeyer J et al. Pyrrolizidine alkaloid-induced alterations of prostanoid synthesis in human endothelial cells. Chem Biol Interact 298:104-111 (2019). PubMed: 30465738
- Li H et al. Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway. Int J Biol Sci 15:2037-2050 (2019). PubMed: 31592228
- Russo A et al. Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma. J Oncol 2018:6301980 (2018). PubMed: 30532780
- Tamosiuniene R et al. Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension. Circ Res 122:1689-1702 (2018). PubMed: 29545367
- Tian L et al. Effects of TSH on the function of human umbilical vein endothelial cells. J Mol Endocrinol 52:215-22 (2014). PubMed: 24444496
- Cipollone F et al. A unique microRNA signature associated with plaque instability in humans. Stroke 42:2556-63 (2011). PubMed: 21817153
- Singh SR et al. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol 299:L169-83 (2010). ICC/IF ; Human . PubMed: 20435685
- Lee EJ & Niklason LE A novel flow bioreactor for in vitro microvascularization. Tissue Eng Part C Methods 16:1191-200 (2010). WB ; Rat . PubMed: 20170423
- Bondesen BA et al. Inhibition of myoblast migration by prostacyclin is associated with enhanced cell fusion. FASEB J 21:3338-45 (2007). ICC/IF ; Mouse . PubMed: 17488951
