Anti-PTBP1 抗体 (ab5642)
Key features and details
- Goat polyclonal to PTBP1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-PTBP1 antibody
PTBP1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PTBP1 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Cow, Dog, Pig
-
免疫原
-
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 61-64 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. -
配列類似性
Contains 4 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 282018 Cow
- Entrez Gene: 5725 Human
- Entrez Gene: 19205 Mouse
- Entrez Gene: 397461 Pig
- Entrez Gene: 29497 Rat
- Omim: 600693 Human
- SwissProt: Q8WN55 Cow
- SwissProt: P26599 Human
see all -
別名
- 57 kDa RNA binding protein PPTB 1 antibody
- 57 kDa RNA-binding protein PPTB-1 antibody
- Heterogeneous nuclear ribonucleoprotein I antibody
see all
画像
-
Western blot - Anti-PTBP1 antibody (ab5642)All lanes : Anti-PTBP1 antibody (ab5642) at 0.01 µg/ml
Lane 1 : Human epithelial cells from cervix adenocarcinoma
Lane 2 : Human liver hepatocellular carcinoma
Lane 3 : Human epithelial cells from embryonic kidney
Lysates/proteins at 35 µg per lane.
Predicted band size: 61-64 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling PTBP1 with ab5642 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling PTBP1 with ab5642 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.
-
Western blot - Anti-PTBP1 antibody (ab5642)ab5642 staining (1
ab5642 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.µ g/ml) of Jurkat lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody (ab5642)ICC/IF image of ab5642 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5642, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)IHC image of ab5642 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5642, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-PTBP1 antibody (ab5642)Anti-PTBP1 antibody (ab5642) at 0.1 µg/ml + Human Ovary Lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 61-64 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)ab5642 (5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining shows nuclear staining of keratinocytes.
プロトコール
参考文献 (16)
ab5642 は 16 報の論文で使用されています。
- Zhang Y et al. Long non-coding RNA ARAP1-AS1 promotes tumorigenesis and metastasis through facilitating proto-oncogene c-Myc translation via dissociating PSF/PTB dimer in cervical cancer. Cancer Med 9:1855-1866 (2020). PubMed: 31953923
- Cho CY et al. PTBP1-mediated regulation of AXL mRNA stability plays a role in lung tumorigenesis. Sci Rep 9:16922 (2019). PubMed: 31729427
- Zhang X et al. LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1. Oncogenesis 8:73 (2019). PubMed: 31822653
- Gupta MK et al. Altered transcriptional regulatory proteins in glioblastoma and YBX1 as a potential regulator of tumor invasion. Sci Rep 9:10986 (2019). PubMed: 31358880
- Fu X et al. Suppression of PTBP1 signaling is responsible for mesenchymal stem cell induced invasion of low malignancy cancer cells. Biochim Biophys Acta Mol Cell Res 1865:1552-1565 (2018). PubMed: 30327198
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com