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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
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Anti-PTBP1 抗体 (ab5642)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (16)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-PTBP1  antibody (ab5642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1  antibody (ab5642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1  antibody (ab5642)
  • Western blot - Anti-PTBP1  antibody (ab5642)
  • Immunocytochemistry/ Immunofluorescence - Anti-PTBP1  antibody (ab5642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1  antibody (ab5642)
  • Western blot - Anti-PTBP1  antibody (ab5642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1  antibody (ab5642)

Key features and details

  • Goat polyclonal to PTBP1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PTBP1 antibody [EPR9048(B)] (ab133734)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-PTBP1 antibody
    PTBP1 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to PTBP1
  • 由来種

    Goat
  • アプリケーション

    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human PTBP1 aa 2-14 (N terminal).
    Sequence:

    DGIVPDIAVGTKR-C


    Database link: P26599
    (Peptide available as ab23105)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human PTBP1 protein (ab131990)

アプリケーション

Our Abpromise guarantee covers the use of ab5642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 61-64 kDa).

1 hour primary incubation is recommended for this product.

ターゲット情報

  • 機能

    Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
  • 配列類似性

    Contains 4 RRM (RNA recognition motif) domains.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession P26599 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 282018 Cow
    • Entrez Gene: 5725 Human
    • Entrez Gene: 19205 Mouse
    • Entrez Gene: 397461 Pig
    • Entrez Gene: 29497 Rat
    • Omim: 600693 Human
    • SwissProt: Q8WN55 Cow
    • SwissProt: P26599 Human
    • SwissProt: P17225 Mouse
    • SwissProt: Q29099 Pig
    • SwissProt: Q00438 Rat
    • Unigene: 172550 Human
    • Unigene: 265610 Mouse
    • Unigene: 472059 Mouse
    • Unigene: 64440 Rat
    see all
  • 別名

    • 57 kDa RNA binding protein PPTB 1 antibody
    • 57 kDa RNA-binding protein PPTB-1 antibody
    • Heterogeneous nuclear ribonucleoprotein I antibody
    • Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody
    • hnRNP I antibody
    • HNRNP-I antibody
    • HNRNPI antibody
    • HNRPI antibody
    • MGC10830 antibody
    • MGC8461 antibody
    • Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody
    • Polypyrimidine Tract Binding Protein 1 antibody
    • Polypyrimidine tract binding protein antibody
    • Polypyrimidine tract-binding protein 1 antibody
    • pPTB antibody
    • PTB 1 antibody
    • PTB 2 antibody
    • PTB 3 antibody
    • PTB 4 antibody
    • PTB antibody
    • PTB T antibody
    • PTB1 antibody
    • PTB2 antibody
    • PTB3 antibody
    • PTB4 antibody
    • PTBP 1 antibody
    • PTBP1 antibody
    • PTBP1_HUMAN antibody
    • PTBT antibody
    • RNA Binding Protein antibody
    see all

画像

  • Western blot - Anti-PTBP1  antibody (ab5642)
    Western blot - Anti-PTBP1 antibody (ab5642)
    All lanes : Anti-PTBP1 antibody (ab5642) at 0.01 µg/ml

    Lane 1 : Human epithelial cells from cervix adenocarcinoma
    Lane 2 : Human liver hepatocellular carcinoma
    Lane 3 : Human epithelial cells from embryonic kidney

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 61-64 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1  antibody (ab5642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)

    Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling PTBP1 with ab5642 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1  antibody (ab5642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)

    Immunohistochemical analysis of paraffin embedded Human Kidney tissue labeling PTBP1 with ab5642 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.

  • Western blot - Anti-PTBP1  antibody (ab5642)
    Western blot - Anti-PTBP1 antibody (ab5642)

    ab5642 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

    ab5642 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunocytochemistry/ Immunofluorescence - Anti-PTBP1  antibody (ab5642)
    Immunocytochemistry/ Immunofluorescence - Anti-PTBP1 antibody (ab5642)
    ICC/IF image of ab5642 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5642, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1  antibody (ab5642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)
    IHC image of ab5642 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5642, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-PTBP1  antibody (ab5642)
    Western blot - Anti-PTBP1 antibody (ab5642)
    Anti-PTBP1 antibody (ab5642) at 0.1 µg/ml + Human Ovary Lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 61-64 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1  antibody (ab5642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab5642)
    ab5642 (5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining shows nuclear staining of keratinocytes.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (16)

    ab5642 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5642 は 16 報の論文で使用されています。

    • Zhang Y  et al. Long non-coding RNA ARAP1-AS1 promotes tumorigenesis and metastasis through facilitating proto-oncogene c-Myc translation via dissociating PSF/PTB dimer in cervical cancer. Cancer Med 9:1855-1866 (2020). PubMed: 31953923
    • Cho CY  et al. PTBP1-mediated regulation of AXL mRNA stability plays a role in lung tumorigenesis. Sci Rep 9:16922 (2019). PubMed: 31729427
    • Zhang X  et al. LncRNA MACC1-AS1 sponges multiple miRNAs and RNA-binding protein PTBP1. Oncogenesis 8:73 (2019). PubMed: 31822653
    • Gupta MK  et al. Altered transcriptional regulatory proteins in glioblastoma and YBX1 as a potential regulator of tumor invasion. Sci Rep 9:10986 (2019). PubMed: 31358880
    • Fu X  et al. Suppression of PTBP1 signaling is responsible for mesenchymal stem cell induced invasion of low malignancy cancer cells. Biochim Biophys Acta Mol Cell Res 1865:1552-1565 (2018). PubMed: 30327198
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PTBP1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (184A1 mammary epithelial)
    Permeabilization
    Yes - PBS + 0.25% Triton-X
    Specification
    184A1 mammary epithelial
    Blocking step
    LI-COR Intercept Blocking Buffeer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 18 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PTBP1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Liver)
    Permeabilization
    Yes - TBS+0.025% triton-X-100
    Specification
    Liver
    Blocking step
    10%NGS+1%BSA (in TBS) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 03 2020

    Question

    Batch number 81167 Problem: No bands Material: Cell extract Loaded: Proteins from 4x 6-well plates (Hela and 293T cells) - probably several hundred micrograms. Control GAPDH and Actin worked well. Cells were immediately lysed with protein sample buffer, boiled, spun and loaded on gel. First Ab (ab5642) 1 hr RT. Second - Goat - 1 hr RT 1:10000 dilution. Western was done 3 times.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2005

    Answer

    Thank you for the details that you have provided. At this point, I would like to make the following suggestions. Use ab5642 at a concentration of 1 µg/ml and incubate overnight at 4C. Do not re-use the primary antibody. Ensure that your secondary antibody is working properly, and you may need to increase the concentration of the secondary antibody as well as the incubation period. Do not overwash the membrane. Also, I suggest running a positive control - Jurkat lysate is recommended as positive control for ab5642.

    Read More

    Abcam Scientific Support

    Answered on Mar 04 2005

    Question

    I ordered Anti-PTBP1 (ab5642) from your company. This antibody does not seem to work. Other control antibodies work fine for me. Can you recommend a secondary antibody (and send me a sample or quote) so I can test it again. I use the Amersham's ECL kit.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 25 2005

    Answer

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab5642. A secondary antibody that is compatible for use with this primary is ab6741 - Rabbit polyclonal to Goat IgG H&L (HRP). I would like to help you out and if you would kindly answer the questions below, the answers will enable us to investigate this matter as quickly as possible. Also, please include the batch number that you received (it is located on the vial) and the Abcam order number or purchase order number that was used. 1. Please describe the problem (high background, wrong band size, more bands, no band etc). 2. On what material are you testing the antibody in WB? •Species? •Cell extract/ Nuclear extract? •Purified protein? •Recombinant protein? 3. How much protein did you load? •How did you prepare the lysate for the analysis (protease inhibitors etc)? •Did you heat the samples? 4. Primary Antibody •Specification (in which species was it raised against)? •At what dilution(s) have you tested this antibody? •Incubation time, wash step? 5. Secondary Antibody •Specification (in which species was it raised against)? •At what dilution(s) have you tested this antibody? •Incubation time, wash step? •Do you know whether the problems you are experiencing come from the secondary? 6. What detection method are you using? 7. Background bands •Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control) •Is the blocking step sufficient? (We recommend blocking the membrane by adding 20 ml of blocking buffer (5% non-fat dry milk, 0.1% Tween-20 in TBS). Incubate for 2 h at room temperature or overnight at 4°C with agitation) •Are your washing steps sufficiently stringent? (Multiple short washes are more effective than fewer longer wash steps) •At what size are the bands migrating? Could they be degradation products of your target? •Please provide an image of your blot (as an e-mail attachment, a faxed image is not sufficient) 8. Optimization attempts •How many times have you tried the Western? •Do you obtain the same results every time e.g. are background bands always in the same place? •What steps have you altered? 9. Did you apply positive and negative controls along with the samples? Please specify.

    Read More

    Abcam Scientific Support

    Answered on Feb 28 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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