Anti-PSMG1 抗体 [EPR10223] (ab167396)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10223] to PSMG1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PSMG1 antibody [EPR10223]
PSMG1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10223] to PSMG1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IPmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- U-87 MG, Jurkat, 293T and HepG2 cell lysates; Human brain and Human testis tissues; HepG2 cells;
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10223 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167396の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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IP |
1/10 - 1/100.
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特記事項 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 33 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
IP
1/10 - 1/100. |
ターゲット情報
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関連性
PSMG1 is a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization. There are two named isoforms. -
細胞内局在
Cytoplasm. Endoplasmic reticulum. -
参照データベース
- Entrez Gene: 8624 Human
- Entrez Gene: 56088 Mouse
- Entrez Gene: 288236 Rat
- Omim: 605296 Human
- SwissProt: O95456 Human
- SwissProt: Q9JK23 Mouse
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別名
- AW552102 antibody
- c21 LRP antibody
- C21LRP antibody
see all
画像
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All lanes : Anti-PSMG1 antibody [EPR10223] (ab167396) at 1/10000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa -
Detection of PSMG1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab167396 at 1/10 dilution.
Anti-PSMG1 antibody [EPR10223] (ab167396) at 1/10000 dilution + Immunoprecipitation pellet from HepG2 cell lysate at 10 µg -
Immunofluorescent analysis of HepG2 cells, labeling PSMG1 with ab167396 at 1/250 dilution.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSMG1 with ab167396 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing PSMG1 with ab167396 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab167396 は 1 報の論文で使用されています。
- Moritz KE et al. The role of the immunoproteasome in interferon-?-mediated microglial activation. Sci Rep 7:9365 (2017). PubMed: 28839214