Anti-PSME1 抗体 [EPR10967(B)] (ab155091)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10967(B)] to PSME1
- Suitable for: Indirect ELISA, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PSME1 antibody [EPR10967(B)]
PSME1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10967(B)] to PSME1 -
由来種
Rabbit -
アプリケーション
適用あり: Indirect ELISA, WB, ICC/IFmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human PSME1. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HEK293T, MCF7 and Jurkat lysates. ICC/IF: MCF7 and HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10967(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155091の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Indirect ELISA |
Use at an assay dependent concentration.
Use at 31.25 ng /ml |
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WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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ICC/IF | (1) |
1/500.
For unpurified use at 1/100 - 1/250. |
特記事項 |
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Indirect ELISA
Use at an assay dependent concentration. Use at 31.25 ng /ml |
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
ICC/IF
1/500. For unpurified use at 1/100 - 1/250. |
ターゲット情報
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機能
Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. -
配列類似性
Belongs to the PA28 family. - Information by UniProt
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参照データベース
- Entrez Gene: 5720 Human
- Entrez Gene: 19186 Mouse
- Entrez Gene: 29630 Rat
- Omim: 600654 Human
- SwissProt: Q06323 Human
- SwissProt: P97371 Mouse
- SwissProt: Q63797 Rat
- Unigene: 75348 Human
see all -
別名
- 11S regulator complex alpha subunit antibody
- 11S regulator complex subunit alpha antibody
- 29 kD MCP activator subunit antibody
see all
画像
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Indirect ELISA analysis of Human Proteasome activator complex subunit 1 using ab155091 at 1000 ng/mL dilution and secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 2500 ng/mL dilution
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All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PSME1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-4: Merged signal (red and green). Green - ab155091 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab155091 Anti-PSME1 antibody [EPR10967(B)] was shown to specifically react with PSME1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266192 (knockout cell lysate ab257613) was used. Wild-type and PSME1 knockout samples were subjected to SDS-PAGE. ab155091 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 29 kDa -
Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.
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All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution
All lanes : Human prostate cancer whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 2 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab155091 は 4 報の論文で使用されています。
- Park N et al. Phosphorylation in Novel Mitochondrial Creatine Kinase Tyrosine Residues Render Cardioprotection against Hypoxia/Reoxygenation Injury. J Lipid Atheroscler 10:223-239 (2021). PubMed: 34095014
- Woodle ES et al. Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells. Am J Transplant 20:399-410 (2020). PubMed: 31595669
- Jimenez-Guardeño JM et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358
- Welk V et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J Biol Chem 291:13147-59 (2016). PubMed: 27129254