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リコンビナントRabMAb

Anti-PSMD14 抗体 [EPR4257] (ab109123)

Reviews (2) Submit a question References (2)
Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (ab109123)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (ab109123)
  • Anti-PSMD14 antibody [EPR4257] (ab109123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4257] to PSMD14
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PSMD14 antibody [EPR4257]
    PSMD14 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4257] to PSMD14

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109123の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).
ICC/IF
1/500 - 1/1000.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).
ICC/IF
1/500 - 1/1000.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.

  • 組織特異性

    Widely expressed. Highest levels in heart and skeletal muscle.

  • 配列類似性

    Belongs to the peptidase M67A family. PSMD14 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 26S proteasome non-ATPase regulatory subunit 14 antibody
      • 26S proteasome regulatory subunit rpn11 antibody
      • 26S proteasome-associated PAD1 homolog 1 antibody
      • 26S proteasome-associated PAD1 homolog antibody
      • PAD1 antibody
      • PAD1, yeast, homolog of antibody
      • POH1 antibody
      • Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody
      • Proteasome 26S subunit non ATPase 14 antibody
      • PSDE_HUMAN antibody
      • Psmd14 antibody
      • RPN11 antibody
      • Testis tissue sperm binding protein Li 69n antibody
      see all

    画像

    • Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)
      Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)
      All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : Namalwa cell lysates
      Lane 5 : K562 cell lysates
      Lane 6 : MCF7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (ab109123)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (ab109123)

      ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (ab109123)
      Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (ab109123)
      ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.
    • Anti-PSMD14 antibody [EPR4257] (ab109123)
      Anti-PSMD14 antibody [EPR4257] (ab109123)

    参考文献 (2)

    ab109123 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109123 は 2 報の論文で使用されています。

    • Sun T  et al. Deubiquitinase PSMD14 promotes ovarian cancer progression by decreasing enzymatic activity of PKM2. Mol Oncol 15:3639-3658 (2021). PubMed: 34382324
    • Sakai W  et al. Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair. Sci Rep 10:19704 (2020). PubMed: 33184426

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-PSMD14 antibody [EPR4257]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (osteosarcoma)
    Gel Running Conditions
    Reduced Denaturing (15)
    Loading amount
    30 µg
    Specification
    osteosarcoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Anita Luu

    Verified customer

    投稿 Aug 25 2021

    Western blot abreview for Anti-PSMD14 antibody [EPR4257]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Purified protein (protein purified from yeast or recombinantly expre)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine Biorad Stain free)
    Loading amount
    0.4 µg
    Specification
    protein purified from yeast or recombinantly expre
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ellen Goodall

    Verified customer

    投稿 Aug 01 2017

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