Anti-PSMD11 抗体 (ab99413)
Key features and details
- Rabbit polyclonal to PSMD11
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PSMD11 antibody
PSMD11 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PSMD11 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human PSMD11 (NP_002806.2).
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ポジティブ･コントロール
- Human HeLa and 293T cell lines. Mouse NIH3T3 cell line.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99413の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
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| IP | (2) |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 47 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity. -
組織特異性
Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate. -
配列類似性
Belongs to the proteasome subunit S9 family.
Contains 1 PCI domain. -
翻訳後修飾
Phosphorylated by AMPK. -
細胞内局在
Nucleus. Cytoplasm > cytosol. - Information by UniProt
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参照データベース
- Entrez Gene: 513461 Cow
- Entrez Gene: 480610 Dog
- Entrez Gene: 36638 Drosophila melanogaster
- Entrez Gene: 5717 Human
- Entrez Gene: 69077 Mouse
- Entrez Gene: 303353 Rat
- Entrez Gene: 100492225 Xenopus tropicalis
- Entrez Gene: 322265 Zebrafish
see all -
別名
- 26S proteasome non-ATPase regulatory subunit 11 antibody
- 26S proteasome regulatory subunit 9 antibody
- 26S proteasome regulatory subunit p44.5 antibody
see all
画像
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Western blot - Anti-PSMD11 antibody (ab99413)All lanes : Anti-PSMD11 antibody (ab99413) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PSMD11 antibody (ab99413)Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99413 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in laneWestern Blot: anti-PSMD11 antibody (ab99413) at 0.4 µg/ml
developed using the ECL technique
Exposure time : 10 seconds
Predicted band size : 47 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab99413 は 1 報の論文で使用されています。
- Fatima A et al. The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells. Commun Biol 3:262 (2020). PubMed: 32451438
