Key features and details
- Rabbit polyclonal to PSMD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Cow, Human, Non human primates
- Isotype: IgG
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab2941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract.
|IHC-P||Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
機能Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
配列類似性Belongs to the proteasome subunit S1 family.
Contains 10 PC repeats.
- Information by UniProt
- MGC133041 antibody
- 26S proteasome non ATPase regulatory subunit 1 antibody
- 26S proteasome non ATPase subunit 1 antibody
Western blot of proteasome Rpn2 from HeLa cell extract using ab2941.
ab2941 (4µg/ml) staining Rpn2 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei and cytoplasm within the sebaceous gland.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab2941 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2941, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab2941 は 3 報の論文で使用されています。
- Bhat K et al. Serum erythropoietin levels, breast cancer and breast cancer-initiating cells. Breast Cancer Res 21:17 (2019). PubMed: 30700319
- Lu X et al. Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Nat Commun 8:15540 (2017). PubMed: 28598414
- Moscovitz O et al. The Parkinson's-associated protein DJ-1 regulates the 20S proteasome. Nat Commun 6:6609 (2015). WB . PubMed: 25833141