Anti-PSMA4 抗体 [EPR5831(2)] (ab191403)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5831(2)] to PSMA4
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PSMA4 antibody [EPR5831(2)]
PSMA4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5831(2)] to PSMA4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- K562, HeLa, Jar, rat C6, rat PC12 and mouse NIH 3T3 cell lysates. Human papillary adenocarcinoma of thyroid and mouse liver tissues. HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5831(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab191403の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/500.
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WB |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/200.
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特記事項 |
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ICC/IF
1/500. |
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/200. |
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
配列類似性
Belongs to the peptidase T1A family. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasm, P-body. Colocalizes with TRIM5 in the cytoplasmic bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 5685 Human
- Entrez Gene: 26441 Mouse
- Entrez Gene: 29671 Rat
- Omim: 176846 Human
- SwissProt: P25789 Human
- SwissProt: Q9R1P0 Mouse
- SwissProt: P21670 Rat
- Unigene: 251531 Human
see all -
別名
- HC9 antibody
- HsT17706 antibody
- Macropain subunit C9 antibody
see all
画像
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PSMA4 was immunoprecipitated from 0.35mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab191403 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab191403 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate, 10µg
Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191403 in K-562 whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Observed MV: 29kDa.
Exposure time: 180 seconds.
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All lanes : Anti-PSMA4 antibody [EPR5831(2)] (ab191403) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa -
All lanes : Anti-PSMA4 antibody [EPR5831(2)] (ab191403) at 1/1000 dilution
Lane 1 : rat C6 cell lysate
Lane 2 : mouse Raw 264.7 cell lysate
Lane 3 : rat PC12 cell lysate
Lane 4 : mouse NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa -
Immunohistochemical analysis of paraffin-embedded, Human papillary adenocarcinoma of thyroid tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling PSMA4 with ab191403 at a 1/500 dilution ( 4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PSMA4 at a 1/500 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab191403 は論文での使用が確認できていません。