Anti-PSD95 (phospho S295) 抗体 [EP2615Y] (ab76108)
Key features and details
- Rabbit monoclonal [EP2615Y] to PSD95 (phospho S295)
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-PSD95 (phospho S295) antibody [EP2615Y]
PSD95 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2615Y] to PSD95 (phospho S295) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human brain tissue treated with LP and rat brain lysates, untreated and treated with LP.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP2615Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76108の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). |
ターゲット情報
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機能
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. -
組織特異性
Brain. -
配列類似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
ドメイン
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density. -
翻訳後修飾
Palmitoylation of isoform 1 is required for targeting to postsynaptic density. -
細胞内局在
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. - Information by UniProt
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参照データベース
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 463928 Human
see all -
別名
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
画像
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All lanes : Anti-PSD95 (phospho S295) antibody [EP2615Y] (ab76108) at 1/20000 dilution
Lane 1 : Human brain tissue lysate, treated with LP
Lane 2 : Human brain tissue lysate untreated
Lane 3 : Rat brain lysate, treated with LP
Lane 4 : Rat brain lysate, untreated
Predicted band size: 95 kDa
Observed band size: 95 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab76108 は 4 報の論文で使用されています。
- Mannara F et al. Allosteric modulation of NMDA receptors prevents the antibody effects of patients with anti-NMDAR encephalitis. Brain 143:2709-2720 (2020). PubMed: 32830245
- Bodrikov V et al. Reggie-1 and reggie-2 (flotillins) participate in Rab11a-dependent cargo trafficking, spine synapse formation and LTP-related AMPA receptor (GluA1) surface exposure in mouse hippocampal neurons. Exp Neurol 289:31-45 (2017). PubMed: 27993509
- Feng Y et al. Neuroprotective effects of resveratrol against traumatic brain injury in rats: Involvement of synaptic proteins and neuronal autophagy. Mol Med Rep 13:5248-54 (2016). PubMed: 27122047
- Zhu YC et al. Palmitoylation-dependent CDKL5-PSD-95 interaction regulates synaptic targeting of CDKL5 and dendritic spine development. Proc Natl Acad Sci U S A 110:9118-23 (2013). PubMed: 23671101