リコンビナントRabMAb

Anti-PSD95 抗体 [EPR23124-118] - BSA and Azide free (ab269863)

Submit a review Submit a question
Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
  • Immunoprecipitation - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
  • Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
  • Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23124-118] to PSD95 - BSA and Azide free
  • Suitable for: IHC-P, WB, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free
    PSD95 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23124-118] to PSD95 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, IHC-Fr, IPmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse brain, Mouse cerebellum, Rat brain, Human cerebellum and Human brain lysates. IHC-P: Mouse cerebellum and Rat retina tissues. IP: Mouse brain lysate. IHC-Fr: Mouse and rat retina tissue.

  • 特記事項

    ab269863 is the carrier-free version of ab238135. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab269863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for ICC/IF.

    • ターゲット情報

    • 機能

      Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.

    • 組織特異性

      Brain.

    • 配列類似性

      Belongs to the MAGUK family.
      Contains 1 guanylate kinase-like domain.
      Contains 3 PDZ (DHR) domains.
      Contains 1 SH3 domain.

    • ドメイン

      The PDZ domain 3 mediates interaction with ADR1B.
      The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.

    • 翻訳後修飾

      Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

    • 細胞内局在

      Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Discs large homolog 4 antibody
      • Disks large homolog 4 antibody
      • DLG 4 antibody
      • Dlg4 antibody
      • DLG4_HUMAN antibody
      • FLJ97752 antibody
      • FLJ98574 antibody
      • Human post synaptic density protein 95 antibody
      • Post synaptic density protein 95 antibody
      • Postsynaptic density protein 95 antibody
      • PSD 95 antibody
      • PSD-95 antibody
      • PSD95 antibody
      • SAP 90 antibody
      • SAP-90 antibody
      • SAP90 antibody
      • Synapse associated protein 90 antibody
      • Synapse-associated protein 90 antibody
      • Tax interaction protein 15 antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
      Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)

      Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat retina tissue labeling VSIG4 with ab238135 at 1/100 (5.76 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238135).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)

      Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling PSD95 with ab238135 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on outer plexiform layer of rat retina. The section was incubated with ab238135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238135).

    • Immunoprecipitation - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
      Immunoprecipitation - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)

      PSD95 was immunoprecipitated from 0.35 mg Mouse brain lysate with ab238135 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238135 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse brain lysate 10µg
      Lane 2: ab238135 IP in Mouse brain lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238135 in Mouse brain lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238135).

    • Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
      Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)

      Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling VSIG4 with ab238135 at 1/100 (5.76 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238135).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling PSD95 with ab238135 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basket cells of mouse cerebellum. The section was incubated with ab238135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238135).

    • Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)
      Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free (ab269863)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269863 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269863 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab269863.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com