Anti-PSD95 抗体 (ab12093)
Key features and details
- Goat polyclonal to PSD95
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-PSD95 antibody
PSD95 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PSD95 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.025% Sodium azide
Constituents: PBS, 1% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12093の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).
Abcam recommends using 3% milk as the blocking agent. |
| IHC-P |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa). Abcam recommends using 3% milk as the blocking agent. |
|
IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
-
機能
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. -
組織特異性
Brain. -
配列類似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
ドメイン
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density. -
翻訳後修飾
Palmitoylation of isoform 1 is required for targeting to postsynaptic density. -
細胞内局在
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. - Information by UniProt
-
参照データベース
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 463928 Human
see all -
別名
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
画像
-
Western blot - Anti-PSD95 antibody (ab12093)Lanes 1-4 : Anti-PSD95 antibody (ab12093) at 2 µg/ml (Blocked with 2% BSA)
Lanes 5-8 : Anti-PSD95 antibody (ab12093) at 2 µg/ml (Blocked with 3% Milk)
Lanes 1 & 5 : Human brain cerebral cortex tissue lysate - total protein (ab30061)
Lanes 2 & 6 : Human brain tissue lysate - total protein
Lanes 3 & 7 : Brain (Mouse) Tissue Lysate
Lanes 4 & 8 : Brain (Rat) Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 38 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin or 3% Milk before being incubated with ab12093 overnight at 4°C. Antibody binding was detected using an anti-goat antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody (ab12093)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdomab12093 PSD95 antibody immunostaining brain tissue (4% PFA perfused paraffin embedded 5 micron saggital section). ab12093 was used at 10 µg/ml for a 20 minute incubation at 37 degrees C (on a Ventana Discovery slide processor). Light antigen retrieval and then light protease treatment is recommended before addition of primary antibody ab12093 in this paradigm. -
Western blot - Anti-PSD95 antibody (ab12093)This image is courtesy of an Abreview submitted by Dr Martin BroadstockAll lanes : Anti-PSD95 antibody (ab12093) at 1/5000 dilution
Lane 1 : rat cortical lysate
Lane 2 : mouse cortical lysate
Lanes 3-8 : BA21 human cortical lane lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexafluor 680
Performed under reducing conditions.
Predicted band size: 80 kDa
See abreview for further details -
Western blot - Anti-PSD95 antibody (ab12093)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomLane 1 : Anti-PSD95 antibody (ab12093) at 0.3 µg/ml
Lane 2 : Anti-PSD95 antibody (ab12093) at 1 µg/ml
Lanes 3-4 : Anti-PSD95 antibody (ab12093) at 3 µg/ml
Lanes 1-3 : 10µg mouse brain extract/lane
Lane 4 : 10µg mouse brain extract/lane with Mouse PSD95 peptide (ab12419) at 1 µg/ml
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: ~105 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsPSD95 (ab12093) western blot on mouse brain extract (10
µ g protein/lane), showing use of the immunising peptide (ab12419) as a control. The blot was exposed for 10 seconds to autoradiographic film.Lane 1 = 0.3
µ
Lane 2 = 1µ
Lane 3 = 3µ
Lane 4 = 3µ µ
Lane 5 = Ladder
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (65)
ab12093 は 65 報の論文で使用されています。
- LaMassa N et al. Gamma-protocadherin localization at the synapse is associated with parameters of synaptic maturation. J Comp Neurol 529:2407-2417 (2021). PubMed: 33381867
- Illiano P et al. Early Adolescence Prefrontal Cortex Alterations in Female Rats Lacking Dopamine Transporter. Biomedicines 9:N/A (2021). PubMed: 33562738
- Ikezu S et al. Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities. Mol Psychiatry 26:1808-1831 (2021). PubMed: 32071385
- Grubman A et al. Transcriptional signature in microglia associated with Aß plaque phagocytosis. Nat Commun 12:3015 (2021). PubMed: 34021136
- Cano JC et al. The amygdala modulates prepulse inhibition of the auditory startle reflex through excitatory inputs to the caudal pontine reticular nucleus. BMC Biol 19:116 (2021). PubMed: 34082731